
3. Please complete the following table with the terms: "a lot, a little," or "none." is...
LOJL B) glucose C) galactose D) fructose 2. Define the term “polycistronic." Make sure you use the terms "promoter," "terminator," "ribosomal binding site," "start codon," and "stop codon” in your answer, a diagram might be helpful as a part of your answer. 3. Please complete the following table with the terms: "a lot," "a little," or "none. is CAP- CAMP complex is repressor bound? is RNA pol bound? i s lac operon transcribed?
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Fill in the blanks in this table with “yes” or “no” for each condition of lac operon regulation. The strain is wild type, with no partial diploidy. The first line is filled in for reference. Please explain your answers! Condition Lactose levels high? lac repressor bound to operator? cAMP levels high? CAP bound to CAP binding site? Transcription at highest level? high glucose no lactose no Yes no no no no glucose high lactose high glucose high lactose no glucose...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
Fill in the blanks in the following sentences by selecting from the drop-down menus. When E.coli cells are grown in the presence of high glucose and high lactose, the lac repressor is not bound to the lac operator, CAMP levels in the cell are high and CAP is mostly bound to the CAP binding site in the lac promoter. Under these conditions, transcription of the lac operon Select When E.coli cells are grown in the absence of glucose and presence...
The lac operon codes for enzymes that break down lactose, but this operon is only used when needed. This includes: 1. When the repressor is bound at the operator 2.When RNA polymerase is bound to the promotor WITHOUT the CAP molecule 3.When the CAP and cAMP molecules are bound to the RNA polymerase and lactose is bound to the repressor 4.When the CAP and cAMP molecules are bound to the RNA polymerase and lactose is NOT bound to the repressor
answer the following questions about lac operon: a) if the lack repressor protein is bound to the operator, are the E.coli metabolized lactose or not? explain your reasoning. b) If the lack repressor protein is bound to the operator and cellular glucose levels are low, is cAMP-CAP complex bound adjacent to the promotor, or not? explain your reasoning. c) if the lack repressor protein is bound to the operator and cAMP - CAP is bound to the CAP site, are...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
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Which of the following statements are true for bacteria grown in the absence of glucose and absence of lactose? Select all that apply. High levels of cAMP CAP bound to CAP binding site Low or no levels of allolactose O High levels of lacZYA mRNA No transcription of lacZYA Lac repressor bound to operator CAP is not bound to binding site O High levels of allolactose Lac repressor...