Question

You set up an experiment to assess kinetic rate constants for enzyme X with substrates A and B. You use 10 M enzyme in all experiments. You may use excel or any other program to help you answer questions about enzyme X. Use the following set of data to answer the following questions: Vo (UM/s) 500 MB 2.2 Vo (uM/s) 50 MB [A] UM 0.8 4.3 1.4 2.7 4.9 5.8 9.8 11.6 13 13.5 6.5 6.7 16. What pattern is displayed for this bisubstrate reaction catalyzed by enzyme X? (2 pts) 17. What conclusion can be made about the mechanism? (3 pts) a. The catalytic complex is EAB b. There are two separate chemistry events c. The mechanism displayed is ping pong d. Substrate A is the leading substrate e. Using Cleland notation, species F is generated in the catalytic cycle

Answer 1

16) VA 2 1,28 0.13 0.5 014 0.25 0.125 0.0625 0.03125 Vrole (Yvo) soum of B 0.45 0.714 0.37 0.102 0.204 0.086 0.077 0.074 0.17 015 0.149 Esso ung [B7 Conc. So This LB Plot of Bisubstate showed that bolt graph intercept each othes which indicates off that it from ott tertiary 0.4+ complex wilheng [AB] o oour 0.37 o Boone 0.21 Oyhan 0.2 0.4 out of 1.0 1.2 1.4 lis 1.8 2.0 so reachis will be like & + A- (EAB] - E+P,+P₂ &B 17) option ila) is cossect, the catalyst complex is EAB.

Hope it's clear..thanks