Question

Draw the complete chemical structure of the fragment of human immunodeficiency reverse transcriptase, IYQEPFKNLK TGKYARMRGA. Indicate bonds that are hydrolyzed by trypsin, chymotrypsin. What is the maximum number of peaks that can be observed in electrospray ionization mass spectrum of this peptide? Estimate the isolectric point of this peptide.

Answer 1

ures that Answer: Remerse transciptase also called RNA abructed DNA polymerase, an enxyme encoded from the genetic material of retrovirusest catalyzes the transcription of retrouurus RNA Photo DNA. → Trykin is a medium size gesbular protein that functions as a pancreatic serine protease This enzyme chydrelyzes bands by cleaning peptides on the catermenal sides of amino aced residues lysine and arginine → out to chymotrysin, cleanes keptide bones selectriely on the cartongeterminal side of the large imporophobic ameno acids such as tryptophan, tyrosine, phenylalanine and notulksin chymotrufksin da is a good enampele methionine of the use of covalent modification as a catalytic strategy ..... Electrospray nanization mass spectroscopy is another important technique used for the analysis of thermally fragile and high moleculan - megut pelyhery. In this method, a solution of the analyte iis pumped through a high voltage cakiliary and the elected dropllt is transfereed into the gaseous ion phase before ba a hass spectrometric andlyzu. > peptides are short polymer to fragements composed from aminoachas each pektides has a cutain amp no acid sequence denoted with a three letter for enample the anno auld alanine is er one letter code abbrenpated as "Ala" es "A"

the charge of the peptides in the solution abends upon the solution acldity anence y a pepude has an annano Seid sequence Ala - sen - Cylu - Leu - Pro (sianine - serin - Columable aroid -Leucine - Praline) then the one leter sequence is AS ELP".