Question

Question Completion Status QUESTIONS A segment of DNA has two restriction sites - I and II (see below). When incubated with restriction enzymes I and II, three fragments will be formed - a, b, and see below. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments? 3 p wwwwwwwwwwwwwww 10 OTTI - SOVITI III C ond Sub and the Se Alle 1900 195 08-3% 4°C MacBook Air

Answer 1

Ans) The correct answer is "B".

Upon restriction enzyme digestion, three fragments are produced namely a,b and c (since there are two restriction sites I and II )

Out of the three fragments you can clearly observe that fragment c is longest (more the size of DNA fragment) , fragment b is shortest (lesser the size of DNA fragment) and fragment a is intermediate length.

Upon gel electrophoresis, the DNA fragment with shortest size will move the farthest or fast in the agarose gel wheras DNA fragment with highest size will move very slowly on the gel. So,the "c" fragment will be in 1st position, "a" fragment will be in 2nd position and "b" fragment (shortest) will move the farthest and thus acquires 3rd position based on the distance migrated on the gel.