Question

To collect data for an ONP standard curve, you need to prepare a series of test tube with ONP stock of known concentration. How much ONP is found in a test tube with 1 mL of 2 mM ONP stock and 5 mL of PO4 buffer? 2x 10-6 mol 5 x 10-6 mol 1x 1015 mol 2x 10-7 mol

Answer 1

Molarity = # of moles / volume of solution in litres

2 × 10^-3 M = x / 1 × 10^-3 L

X = 2 × 10^{-6 .}

so , there will be 2 × 10-6 moles of ONP in given ONP stock and 5ml of PO4 buffer.