Question

Please help me construct a Ferguson Plot for the following 10% gel

A for Lysozyme is A=0.85714 --> logU(0.85714)

A for RNAse A is A= 0.9657 --> LogU(0.9657)

A for BSA is 0.3985 --> log U (0.3985)

6.76 cm 7 cm 10% gel

Answer 1

The red dotted line is the trendline from we analyse the desired protein mol. wt

0.96 1. 000 .85 Mw(in Da) Rf value (log) ........ Linear 0.10 RNAse 13.7 0.96 Lysozyme 14.3 0.85 BSA 66.6 0.39