Question

Please help me construct a Ferguson plot for a 12% gel concentration containing Lysozyme
Enzyme MW 02 BSA 66-5K) (2) Lysozyme 14.3 Ra (3) RNase A 13.732

the distance for lysozyme is 5.21cm

the distance for BSA is 1.35cm

the distance for RNAse A is 5.58cm

total distance of gel is 7cm

electric field must be approximately E = (100 V)/(20 cm) = 5 V/cm = 500 V/m.

Answer 1

The Ferguson plot can be created by the measurement of mobility.

The mobility can be measured by the formulae

Mobility measurement = Migration velocity (cm/s)/field strength (V/cm) it should be for samples and standards at various gel concentrations.

Further the plot can be generated by plotting log (mobility) vs gel concentration (%T)

The %T at every point on the gradient gel was calculated by

y=0.3997 +0.0518*x .where y is %T and x the distance (cm).

Voltage of the pore gradient gels homogeneous control gels, measured at hourly intervals,

T he curve is described by the function

  y = 1.957 + 0.678-5 . t - 0.733-10 . t2

where y is the average field strength (V/cm) and t is time (s)

.Hence The Ferguson plot was calculated for each samples as log10 (average mobility) vs. average %T

The Ferguson plot was computed on the basis of an input using program FPLOT.BAS

Please apply the values on the given formulae and compute Ferguson plot for your research