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Compare the activities of L-lactate (normal substrate) at - 1mM NAD concentration to the activities of D-lactate (enantiomer
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The enzymes responsible for the conversion of pyruvate to lactic acid and back are lactate dehydrogenases (LDHs). NAD-dependent L-lactate dehydrogenases are responsible for the synthesis of L-lactic acid, whereas D-lactic acid can be produced in either of two ways: through NADH-dependent reduction of pyruvate by NAD-dependent D-lactate dehydrogenases or through racemization of L-lactic acid by an L-lactic-acid-inducible lactate racemase.

cytosolic L-lactate is converted to pyruvate by L-lactate dehydrogenase (LDH), an enzyme using NAD as a cofactor, which subsequently enters into TCA cycle for further energy production. Where as L-malate dehydrogenase is an enzyme in the citric acid cycle that catalyzes the reversible conversion of L-malate into oxaloacetate by the help of its coenzyme NAD+. Malate dehydrogenase catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH.

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