Describe the process by which increased cytosolic cAMP could result in altered gene expression.
Activators enhance the interaction between RNA polymerase and a particular promoter, encouraging the expression of the gene. Activators do this by increasing the attraction of RNA polymerase for the promoter, through interactions with subunits of the RNA polymerase or indirectly by changing the structure of the DNA.
Describe the process by which increased cytosolic cAMP could result in altered gene expression.
Describe the process through which activation of the trimeric G protein, Go, results in increased cytosolic calcium
Describe a way in which a membrane bound signal receptor protein could directly regulate gene expression at the level of transcription activation. If there was an intermediate enzyme in this pathway, what effect could that have?
Discuss mechanisms by which gene expression may be altered. How do these alterations induce cancer-causing mutations in cell DNA? Explain how cancer is formed.
Regulation of Gene Expression Describe the process of cell determination and differentiation in a future skeletal muscle cell.
3. What would be the result in this process of a genetic mutation that altered the shape of the trp repressor protein? (4 points)
Describe how the expression of this gene is regulated. Include
the roles of all key players (GAL3, GAL4, GAL80, Gene promoter, RNA
polymerase, and transcription factors) illustrated.
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down lactose in yeast is regulated. The top image illustrates the key regulators of the process. The bottom two panels illustrate what happens in the presence and absence of galactose. Describe how the expression...
In eukaryotic cells, there are specific transcription factors (activators) that regulate gene expression. Describe the organization of eukaryotic genes (i.e. promoters/enhancers) and how transcription factors are able to control gene expression and enzyme activity. a) Throughout the course we discussed the enzyme lactase and how it is differently regulated (and therefore expressed) in different human populations. Describe how this gene is differently expressed in different populations and when this/these change(s) are predicted to have taken place. b) Differential gene expression...
With diagrams, describe two different mechanisms in which gene expression is controlled at the translational and post-translational levels. You must cite an example for each of these control mechanisms.
Describe how the Lac repressor and the CAP-cAMP complex regulate the expression of the Lac operon in E. coli.
You note that stimulation of a cell with a specific hormone leads to increased expression of a certain gene. You also find that the hormone effect can be inhibited with pertussis toxin. Describe the pathway from hormone to gene that is responsible for this process (a schematic is preferable). Indicate where in your pathway and how/why pertussis toxin has its effect.