Homework Answers
13. Human serum albumin has a negative charge at pH 7.4 and its isoelectric point is 5. Hence when Human serum albumin is placed in an acidic medium it tends to acquire a positive charge and moves towards the negative electrode.
14. BSA is positively charged under acidic conditions and so it would move towards the negative electrode when placed in an agarose gel.
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13) The following is the amino acid sequence of human serum albumin: DAHKSE VAHRFKDLGE ENFKALVLIA FAQYLOQCPF...
The following is the amino acid sequence of human serum albumin: DAHKSE VAHRFKDLGE ENFKALVLIA FAQYLQQCPF EDHVKLVNEV...
The following is the amino acid sequence of human serum albumin: DAHKSE VAHRFKDLGE ENFKALVLIA FAQYLQQCPF EDHVKLVNEV TEFAKTCVAD ESAENCDKSL HTLFGDKLCT VATLRETYGE MADCCAKQEP ERNECFLQHK DDNPNLPRLV RPEVDVMCTA FHDNEETFLK KYLYEIARRH PYFYAPELLF FAKRYKAAFT ECCQAADKAA CLLPKLDELR DEGKASSAKQ RLKCASLQKF GE RAFKAWAV ARLSQRFPKA EFAEVSKLVT DLTKVHTECC HGDLLECADD RADLAKYICE NQDSISSKLK ECCEKPLLEK SHCIAEVEND EMPADLPSLA ADFVESKDVC KNYAEAKDVF LGMFLYEYAR RHPDYSVVLL LRLAKTYETT LEKCCAAADP HECYAKVFDE FKPLVEEPQN LIKQNCELFE QLGEYKFQNA LLVRYTKKVP QVSTPTLVEV SRNLGKVGSK CCKHP EAKRM PCAEDYLSVV LNQLCVLHEK TPVSDRVTKC CTESLVNRRP CFSALEVDET YVPKEFNAET FTFHADICTL SEKERQIKKQ TALVELVKHK PKATKEQLKA VMDDFAAFVE KCCKADDKET CFAEEGKKLV AASQAALGL (BSA is similar to this with 583 amino...
Human Serum Albumin contains 585 amino acids, more than 50 of which are Lysine. If the pl of HSA is about 4.5, what...
Human Serum Albumin contains 585 amino acids, more than 50 of which are Lysine. If the pl of HSA is about 4.5, what do we know about the content of Asp and Glu? >50 O <50 O -50 O none of the above
Human Serum Albumin contains 585 amino acids, more than 50 of which are Lysine. If the pl of HSA is about 4.5, what do we know about the content of Asp and Glu? >50 O
Which of the following describes the pI of an amino acid? Select one: Oa. the pH...
Which of the following describes the pI of an amino acid? Select one: Oa. the pH at which the amino acid is primarily in the zwitterion form b. the pH at which there are equal concentrations of protonated and neutral forms c, the negative log of the concentration of the ionized amino acid d. the pH of the buffer solution used in electrophoresis that causes the amino acid to migrate to the positive electrode 0 O
QU. 5. You have identified the complete amino acid sequence on protein sequencing techniques. "Y" is...
QU. 5. You have identified the complete amino acid sequence on protein sequencing techniques. "Y" is composed of 44 amino acids with the po below. No information is available about its genetic sequence. Your task sequence for protein "Y". Assume that the gene sequence is unique in the human 5 BRIEFLY EXPLAIN methodically and accurately how you will decipher the gene seg "Y". (3 POINTS). no acid sequence of a human protein called "Y" using 1.44 amino acids with the...
The following is a portion of the amino acid sequence of human GLUT1 purified from red...
The following is a portion of the amino acid sequence of human GLUT1 purified from red blood cells. Glucose is also shown for your convenience. 147 151 161 171 181 vspt alrgalgtlh qlgivvqili aqvfgldsim gnkd A. An internal 23 amino acid region of the sequence above is the 5th transmembrane domain of GLUT1. The beginning of the sequence is an intracellular loop. The end of the sequence is an extracellular loop. Indicate where these regions might be in the sequence. Explain how you derived at your hypothesis....
15. Determine the amino acids following mRNA molecules. ne the amino acid sequence a ribosome would...
15. Determine the amino acids following mRNA molecules. ne the amino acid sequence a ribosome would translate from the *GCACCAUGCAAAGCGGGGAUUAGACCUUU-3 Caution: from which end of the RNA strand does the ribosome begin translating? 3-AGAGUCCAUGCAAAGCGGGGAUUGUACCUUU - 5' 16. Determine the amino acid sequence a ribosome would translate from the following non template DNA strand. (Hint: you must first convert the non template DNA strand to a template DNA strand and then to mRNAJ 3'-ACCTTGATCCTTGACGTATGTAGTATGTATC-5'
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that...
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that starts with Methionine. 5- TTATTCTTTAAT CAT-3 What would be the consequences of the highlighted A to be mutated to a G? Select one: a. The resulting peptide will likely be larger than the non-mutant peptide b. the primary sequence of the peptide will be different on one amino acid O C. The peptide sequence would be the same d. all the amino acids after...
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that...
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that starts with Methionine. 5'- TTATTCTTTAAT CAT-3' What would be the consequences of the highlighted A to be mutated to a G? Select one: a. the primary sequence of the peptide will be different on one amino acid b. all the amino acids after the mutation will be different than the non-mutant peptide c. The peptide sequence would be the same d. The resulting peptide...
Exercise 3: Rapid Chromatographic immunoassay for Human Chorionic Gonadotropin (hCG) in Serum or Urine Purpose The...
Exercise 3: Rapid Chromatographic immunoassay for Human Chorionic Gonadotropin (hCG) in Serum or Urine Purpose The student will perform a qualitative test for pregnancy using a rapid chromatographic immunoassay test methodology for the detection of Human Chorionic Gonadotropin (hCG). Human chorionic gonadotropin (hCG) hCG is a glucoprotein hormone comprised of two subunits: alpha and beta (B). The alpha subunit is similar in amino acid sequence to other hormones such as FSH, LH and TSH. The B subunit is not similar...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
Human Serum Albumin contains 585 amino acids, more than 50 of which are Lysine. If the pl of HSA is about 4.5, what do we know about the content of Asp and Glu? >50 O <50 O -50 O none of the above
Human Serum Albumin contains 585 amino acids, more than 50 of which are Lysine. If the pl of HSA is about 4.5, what do we know about the content of Asp and Glu? >50 O
Which of the following describes the pI of an amino acid? Select one: Oa. the pH at which the amino acid is primarily in the zwitterion form b. the pH at which there are equal concentrations of protonated and neutral forms c, the negative log of the concentration of the ionized amino acid d. the pH of the buffer solution used in electrophoresis that causes the amino acid to migrate to the positive electrode 0 O
QU. 5. You have identified the complete amino acid sequence on protein sequencing techniques. "Y" is composed of 44 amino acids with the po below. No information is available about its genetic sequence. Your task sequence for protein "Y". Assume that the gene sequence is unique in the human 5 BRIEFLY EXPLAIN methodically and accurately how you will decipher the gene seg "Y". (3 POINTS). no acid sequence of a human protein called "Y" using 1.44 amino acids with the...
15. Determine the amino acids following mRNA molecules. ne the amino acid sequence a ribosome would translate from the *GCACCAUGCAAAGCGGGGAUUAGACCUUU-3 Caution: from which end of the RNA strand does the ribosome begin translating? 3-AGAGUCCAUGCAAAGCGGGGAUUGUACCUUU - 5' 16. Determine the amino acid sequence a ribosome would translate from the following non template DNA strand. (Hint: you must first convert the non template DNA strand to a template DNA strand and then to mRNAJ 3'-ACCTTGATCCTTGACGTATGTAGTATGTATC-5'
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that starts with Methionine. 5- TTATTCTTTAAT CAT-3 What would be the consequences of the highlighted A to be mutated to a G? Select one: a. The resulting peptide will likely be larger than the non-mutant peptide b. the primary sequence of the peptide will be different on one amino acid O C. The peptide sequence would be the same d. all the amino acids after...
The following sequence is part of the DNA TEMPLATE for a 4 amino acid peptide that starts with Methionine. 5'- TTATTCTTTAAT CAT-3' What would be the consequences of the highlighted A to be mutated to a G? Select one: a. the primary sequence of the peptide will be different on one amino acid b. all the amino acids after the mutation will be different than the non-mutant peptide c. The peptide sequence would be the same d. The resulting peptide...
Exercise 3: Rapid Chromatographic immunoassay for Human Chorionic Gonadotropin (hCG) in Serum or Urine Purpose The student will perform a qualitative test for pregnancy using a rapid chromatographic immunoassay test methodology for the detection of Human Chorionic Gonadotropin (hCG). Human chorionic gonadotropin (hCG) hCG is a glucoprotein hormone comprised of two subunits: alpha and beta (B). The alpha subunit is similar in amino acid sequence to other hormones such as FSH, LH and TSH. The B subunit is not similar...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
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