Question

II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey - one band a) Draw the results on the gel (group your shapes with the gel image, so nothing jumps) b) Are all these single bands of the same size? Briefly explain why yes or why not. c) If you saw two bands for Alexandra and Anastasia, how would you explain that? bp Ladder 1 2 3 4 5 6 7000 4500 4000 2300 2000 1200 1000 800 600 500

Answer 1

Answer 4a:)

The image contains the bands of the gel. In this gel, each student will get the band of the same size because it is PCR products in which each student would have the same sample.

bp Ladder 1 2 3 4 5 6 7000 4500 4000 2300 2000 1200 1000 800 600 500

Answer 4b:)

The PCR process makes copies of the sample. Therefore, each PCR product will have the same size if each student gets the same sample.

Answer 4c:)

The PCR products of Alexandra and Anastasia might have produced by the nonspecific binding of primers. This makes different types of bands.

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