Question

The technique known as 'chromosome painting' in which a single chromosome is painted a unique fluorescent color by base pairing it to labelled pieces of complementary Various chromosome rearrangements such as deletion, duplication, inversion, and translocation of these which of these rearrangements would show up as multicolored chromosomes?

Answer 1

In situ hybridization methods are being used to see the DNA (visualization of chromosomes under microscope). Different probes can be used in this method to see the chromosomes (using fluorescent probes) particular any sort of chromosomal aberration like additon, deletion, inversion or translocation. In situ hybridization, can be visualized using a fluorescent microscope or confocal microscope, which provides an excellent image quality under laser light (red, blue and green laser light). This method can be used for detection of RNA sequences also.