When preparing 250 mL of antibody that is diluted 1:2,000, microliters of antibody would be added...

Question

Question

science biology

Answer 1

Answer #1

Answer

1: 2000 dilution means 1 microliter in 2 ml

so for 250 ml = 250/2= 125 ul

Therefore, 125 microlitres antibody would be added to 250ml of TBST to get the 1:2000 dilution.

Answer 2

Similar Homework Help Questions

17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the...

17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
A 10.00-g sample that contains an analyte is transferred to a 250-mL volumetric flask and diluted...

A 10.00-g sample that contains an analyte is transferred to a 250-mL volumetric flask and diluted to volume. When a 10.00 mL aliquot of the resulting solution is diluted to 25 ml it gives a signal of 0.235 (arbitrary units). A second 10.00 mL portion of the solution is spiked with 10.00 mL of a 1.00-ppm standard solution of the analyze and diluted to 25.00 mL. The signal for the spiked sample is 0.502. Calculate the weight percent of analyte...
if 250 ml of a 1/800 (v/v) solution is diluted to 1 liter what will be...

if 250 ml of a 1/800 (v/v) solution is diluted to 1 liter what will be the ratio strengt

If a solution of 10 mg/ml methyl orange is diluted 100 times, 1 ml added to...

If a solution of 10 mg/ml methyl orange is diluted 100 times, 1 ml added to 99 ml of water for example, what is the final concentration? A. 1 mg/ml B. 0.1 mg/ml C. 0.001 mg/ml
Would the following solutions provide an effective buffer. a) 250. mL of 0.15 M HC2H3O2 +...

Would the following solutions provide an effective buffer. a) 250. mL of 0.15 M HC2H3O2 + 25 mL of 0.10 M NaOH yes no b) 125 mL of 0.50 M HNO2 + 75 mL of 0.40 M KNO2 yes no c) 250. mL of 0.25 M HCO2H + 125 mL of 0.50 M KOH yes no d) 250. mL of 0.50 M HClO4 + 150. mL of 0.50 M NaClO4 yes no e) 250. mL of 0.50 NaOH + 500....
Calculate % v/v when 35 mL ethanol is added to enough water to make 250 mL...

Calculate % v/v when 35 mL ethanol is added to enough water to make 250 mL solution.

If a culture containing 1500 cells/mL is diluted 1/500, what would the final concentration be?

If a culture containing 1500 cells/mL is diluted 1/500, what would the final concentration be?
Vmax = 3.423 µM/sec and enzyme concentration is 250 ug/mL how do I Calculate Kcat? the...

Vmax = 3.423 µM/sec and enzyme concentration is 250 ug/mL how do I Calculate Kcat? the enzyme is alkaline phosphatase and its MW is 140 kDa Additional information: Alkaline phosphatase: 500 µg/mL stock solution in reaction buffer. # [Enzyme] ug/mL Alkaline phosphatase (µL) Buffer (µL) Total volume (µL) 1 100 200 1800 2000 2 125 250 1750 2000 3 150 300 1700 2000
How many moles of potassium hydroxide would have to be added to 250 mL of a...

How many moles of potassium hydroxide would have to be added to 250 mL of a 0.478 M nitrous acid solution, in order to prepare a buffer with a pH of 2.900?

How many moles of sodium hydroxide would have to be added to 250 mL of a...

How many moles of sodium hydroxide would have to be added to 250 mL of a 0.422 M hypochlorous acid solution, in order to prepare a buffer with a pH of 7.360? moles