Question

14) What is the effect on O₂ consumption in a fully functioning mitochondrial suspension that is synthesizing ATP, if the F1 subunit of ATPase is sheared off, leaving an intact Fo subunit? Explain the answer.

Answer 1

There is no ATP synthesis occur as F1 subunit is removed but flow of proton from intermembrane space to matrix will be continues as it's iass through F0 subunit which is intact.

F1 having ATP synthase enzyme present which cause phosphorylation of ADP to ATP once proton fall from intermembrane space to matrix through F0 subunit of Fo-F1 complex.

Hope it's clear.. thanks