15) What is the effect on O2 consumption when venturicidin is added to a fully functioning...
15) What is the effect on O2 consumption when venturicidin is added to a fully functioning mitochondrial suspension that is actively synthesizing ATP? Explain the answer
Homework Answers
- It lead to inhibition of respiration.inhibition was relived by low concentration of uncoupling agents
-Venturicidin is a potent inhibitor of bacterial and mitochondrial ATP synthesis complexes acting on tne F0 membrane sector, with experiments suggesting that the compound strongly inhibits ATP hydrolysis.
-Mechanism of action : Venturicidin binds to the subunit-C of the coupling factor-O (F0) of mitochondrial and bacterial ATP synthese complex.Once bound to the ATP synthase complex, venturicidine blocks proton translocation and inhibits ATP synthesis
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15) What is the effect on O2 consumption when
venturicidin is added to a fully functioning...
14) What is the effect on O2 consumption in a fully functioning mitochondrial suspension that is...
14) What is the effect on O2 consumption in a fully functioning mitochondrial suspension that is synthesizing ATP, if the F1 subunit of ATPase is sheared off, leaving an intact Fo subunit? Explain the answer.
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Consider what will happen to a cell if dinitrophenol (DNP) is added to cause a partial dissipation of the electrochemical gradient across the mitochondrial inner membrane, reducing the gradient from 200 mV to 100 mV. a. Will oxygen consumption increase or decrease? Will proton pumping increase or decrease? Explain. b. Will the ratio of ATP to ADP in the mitochondria increase or decrease? Explain. c. Although it is now banned due to the dangers involved, in the past DNP was...
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