Question

12. Check the best answer in which the drugs on top block the processes below. There may be a case where there is no correct answer. (15 points) Process: Cytochalasin D Bafilomyin Gentamicin Phagocytosis Diphtheria toxin activity Cholera toxin activity ロロロロ ロロロロ ロロロロロ Yersinia invasion Yersinia-induced cell rounding

Answer 1

Process: Cytochalasin D Bafilomyin Gentamicin Phagocytosis Diphtheria toxin activity Cholera toxin activity 00000 Yersinia invasion Yersinia-induced cell rounding

1). Cytochalasins have been shown to inhibit cell phagocytosis and ruffling. In purified actin, cytochalasins have been shown to decrease the amount of F-actin by capping the fast-growth end of actin filaments. 2). (a) On diphtheria toxin fragment A release into the cytosol-Cytochalasin D effect and involvement of actin filaments and eukaryotic elongation factor 2 (b) The cytotoxic action of diphtheria toxin and its degradation in intact Vero cells are inhibited by bafilomycin Al, a specific inhibitor of vacuolar-type H(+) ATPase. c) gentamicin disturbs mitochondrial function and activates apoptosis. 3). Al neutralizes endosomal acidification by inhibiting the vacuolar ATPases responsible for maintaining proton gradients Bafilomycin. Gentamicin competitively binding to cholera toxin (CT) and block its activity. 4). Yersinia is sensitive to Cytochalasins and gentamicin. Toxin translocation is blocked by the use of bafilomycin Al, which specifically inhibits the endosomal proton pump and thereby blocks acidification of endosomes.