8. Two approaches for correcting single-gene defects are gene therapy such as is discussed in Section 3-5D (Base Excision Repair, Nucleotide Excision Repair, and Mismatch Repair) and the CRISPR–Cas9 system. Explain why the CRISPR–Cas9 approach can potentially provide more complete restoration of normal tissue function than gene therapy. (5pts)
Answer:
The reasons why the crispr is preferred over gene therapy are the following
- Crispr can be effectively used in the embryos to delete or knock down any gene that is responsible for formation of any malfunctioned protein. This can change a unhealthy embryo to a completely healthy one . However no matter how much is tried in gene therapy it won't be so effective like that. so Crispr can be used for germline correction of genes.
The reason for this preferability is the precise deletion and insertion of desired DNA into the host genome with the help of crispr cas9 mechanism. Unlike gene therapy they are not random ,hence they don't induce unwanted mutations and hence complications. Moreover there are wide range of genome editing in comparison to gene therapy. So Crispr is preferred for gene correction. It's also found to be more efficient thogh it's not much applicable on humans. China have already been successful in elimination of HIV by this mechanism. Give a thumbs up if you like the answer. Thank you
8. Two approaches for correcting single-gene defects are gene therapy such as is discussed in Section...