Question

Help! Calculate the concentration of lysozyme activity if 150uL of enzyme is added to 850uL of substrate to yield a) -0.0435abs/min at A450? b) 0.000abs/min at A450?

The blank used was water...abs/min=0.000.
df is 20

Answer 1

given,

volume of enzyme = 150 uL

volume of substrate = 850uL

df = 20

A) absorbance/min = -0.0435 abs/min

lysozyme enzyme activity = (absorbance per min/ df) / ( volume of enzyme solution in uL)

= (-0.0435 x 20) / ( 150/850)

= - 4.93

B) absorbance/ min = 0.000 abs/min

lysozyme enzyme activity = (0.000 x 20) / (150/850)

= 0