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Will the BCA Assay allow you to determine the concentration of LDH specifically or the total...

Will the BCA Assay allow you to determine the concentration of LDH specifically or the total protein concentration in your sample? Explain your answer.

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Answer #1

No, BCA assay will not allow you to determine the concentration of LDH specifically rather it allows to determine the total protein concentration in the sample.

The principle of this assay include the reaction between protein and Cu2+ ion. The protein reduce the Cu2+ ion into Cu+ ion in alkaline medium which forms purple color with bicinchoninic acid. The total protein concentration is determined by color change of the sample solution from green to purple in relation to protein concentration. The intensity of the color is measured by using colorimetric techniques.

Since, color change depends on total protein concentration it is not specific to LDH only.

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