Why does the technician PCR add doxycycline to the media?
Why does the technician PCR add doxycycline to the media?
Homework Answers
Doxycycline is an antibiotic which belongs to the class of tetracyclines. It is added in nutrient media plate as a selective agent.
Transformed bacteria which have taken the plasmid contain a gene which imparts resistance to doxycycline in the bacteria. So the addition of doxycycline helps in the selection of transformed bacteria against non transformed bacteria which has not taken up the plasmid.
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does E.coli grow better on LB media or minimal media? please provide an explanation why.
does E.coli grow better on LB media or minimal media? please provide an explanation why.
What does the enzyme exonuclease I do? Why did you treat your initial PCR product with...
What does the enzyme exonuclease I do? Why did you treat your initial PCR product with exonuclease I before using the DNA as a template for Nested PCR? What would have been the result if you had not done an exonuclease I treatment?
• 4. How many copies of the primers do you add to the PCR? You add...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
Why do we add fetal bovine serum to cell culture media? (2-3 paragraphs)
Why do we add fetal bovine serum to cell culture media? (2-3 paragraphs)
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR...
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR deletes DNA b. Polymerase Copying Repeats; PCR amplifies DNA c. Polymerase Copying Releats; PCR deletes DNA d. Polymerase Chain Reaction; PCR amplifies DNA 2) During gel electrophoresis, the DNA fragments are separated by ____ a. charge b. DNA fragments cannot be separated c. color d. size 3) Primers are a. double stranded DNA oligonucleotide (fragment) b. double stranded RNA oligonucleotide (fragment) c. single stranded...
Why is PCR required before running the DNA on a gel? A Without PCR, there would...
Why is PCR required before running the DNA on a gel? A Without PCR, there would be things in the DNA that would interfere with running it on the gel. B Without PCR, there would be too little of the DNA region of interest to see it on the gel. C Without PCR, the DNA region of interest would not be present because PCR adds the part that will be examined. D Without PCR, the gel would not have a...
1. What are some drawbacks to PCR? 2. Why is it necessary to clean up PCR...
1. What are some drawbacks to PCR? 2. Why is it necessary to clean up PCR prodcuts prior to sequencing?
what is the average salary for Patient care technician in Arizona? Does Patient care technician require...
what is the average salary for Patient care technician in Arizona? Does Patient care technician require continuing education units? how many? is there any additional certification required within Patient care technician?
Why do we want/need the presence of Mg2+ in a PCR but do not want it...
Why do we want/need the presence of Mg2+ in a PCR but do not want it present at all other steps of processing/analyzing a DNA sample? (2 marks) Identify and explain what generally occurs in each of the 3 main steps of a polymerase chain reaction. (6 marks) 8. Identify and explain what the 3 different PCR controls inform you about the results of a PCR. (3 marks) Highlight the 2 advantages and 3 disadvantages of using PCR. Ensure to...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
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