Question

What enzymes are added to a sample during RNA isolation to obtain pure RNA? A. DNase...

What enzymes are added to a sample during RNA isolation to obtain pure RNA?

A. DNase only

B. Lysozyme only

C. RNase only

D. Lysozyme and RNase

E. DNase, and Lysozyme

F. RNase and DNase

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Answer #1

Presence of DNA is the main contamination during RNA isolation. The DNA is degraded by using DNase enzyme. Since we are isolating RNA we can't use RNase. Lysozyme are used to degrade bacterial cell wall.

So option A (DNase only) is correct

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