What enzymes are added to a sample during RNA isolation to obtain pure RNA? A. DNase...
What enzymes are added to a sample during RNA isolation to obtain pure RNA?
|
A. DNase only |
||
|
B. Lysozyme only |
||
|
C. RNase only |
||
|
D. Lysozyme and RNase |
||
|
E. DNase, and Lysozyme |
||
|
F. RNase and DNase |
Homework Answers
Presence of DNA is the main contamination during RNA isolation. The DNA is degraded by using DNase enzyme. Since we are isolating RNA we can't use RNase. Lysozyme are used to degrade bacterial cell wall.
So option A (DNase only) is correct
Add Answer to:
What enzymes are added to a sample during RNA isolation to
obtain pure RNA?
A. DNase...
10.During the plasmid isolation procedure we used an enzyme called RNAase. Does RNAase bind RNA specifically...
10.During the plasmid isolation procedure we used an enzyme called RNAase. Does RNAase bind RNA specifically or non-specifically? Why do we use RNAase? If instead of adding RNAase to the sample, what would happen if we added DNase?
Need help with number 1,5,6,7 and 8 please Restriction enzymes are a.) bacterial enzymes that elongate...
Need help with number 1,5,6,7 and 8 please
Restriction enzymes are a.) bacterial enzymes that elongate DNA b.) viral enzymes that destroy host DNA c.) bacterial enzymes that destroy incoming foreign DNA d.) animal enzymes that splice RNA Mice that are injected with only the R strain of Streptococcus pneumoniae a.) become sick and then recover b.) stay healthy, this is a non-virulent strain c.) are killed, this is the virulent strain Mice that are injected with only the S...
What happens if the wrong nucleotide is added during RNA transcription?
What happens if the wrong nucleotide is added during RNA transcription?
(A) During this lab, an organic compound will be added to a pure solvent and you...
(A) During this lab, an organic compound will be added to a pure solvent and you will measure the freezing point of both the solvent and solution. What is your hypothesis regarding the freezing point of the solution relative to the solvent? A hypothesis needs to be focused and testable a. The mass or organic compound will go down as a function of temperature b. The temperature of the freezing point will go up as a function of increasing organic...
You are given a sample of RNA diluted 1:5. You read the OD260 for be 0.45....
You are given a sample of RNA diluted 1:5. You read the OD260 for be 0.45. How much of the non-diluted RNA would be needed to obtain 5ug (micro-grams) of total RNA? A. 5 ml B. 111 ul C. 11.1 ul D. 55.5 ul E. 1.1 ul
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
4. Telomerases... a) are RNA-containing enzymes involved in replicating the ends of linear chromosomes b) cause...
4. Telomerases... a) are RNA-containing enzymes involved in replicating the ends of linear chromosomes b) cause aging in cells c) are structures located at the ends of eukaryotic chromosomes d) are needed by bacteria to complete synthesis of their chromosome ends e) are needed by bacteria to supercoil DNA 5. DNA methylation a) Modifies bases of tRNA molecules, allowing wobble b) Alters the activity of DNA polymerase by affecting access to the DNA c) Is a modification of histone tails...
In order to replicate RNA viruses such as COVID19 must first be converted to DNA. What...
In order to replicate RNA viruses such as COVID19 must first be converted to DNA. What viral enzyme would be an attractive drug target to inhibit this step? A. COVID spike protein B. RNase C. DNA polymerase D. Reverse Transcriptase.
A 30.5 g sample of pure metal at 156.7˚C is added to a 0.700 kg of...
A 30.5 g sample of pure metal at 156.7˚C is added to a 0.700 kg of water (specific heat capacity = 4.180 J/˚C g) at 23.0 ˚C. What is the final temperature of the water if the metal is (a) antimony, specific heat capacity of 0.210 J/˚C g (b) iridium, specific heat capacity of 1.30 J/˚C g? Assume all heat is conserved.
QUESTION 5 Match these terms to the descriptions below. b. , is added onto mRNA during...
QUESTION 5 Match these terms to the descriptions below. b. , is added onto mRNA during processing d , proteins that help initiate RNA synthesis a. spliceosome b. cap c. telomere d. transcription factors e. promoter a, removes introns from primary RNA transcript TATA-rich DNA sequence indicating the start site of a gene c repeating sequences found at the ends of linear DNA
10.During the plasmid isolation procedure we used an enzyme called RNAase. Does RNAase bind RNA specifically or non-specifically? Why do we use RNAase? If instead of adding RNAase to the sample, what would happen if we added DNase?
Need help with number 1,5,6,7 and 8 please
Restriction enzymes are a.) bacterial enzymes that elongate DNA b.) viral enzymes that destroy host DNA c.) bacterial enzymes that destroy incoming foreign DNA d.) animal enzymes that splice RNA Mice that are injected with only the R strain of Streptococcus pneumoniae a.) become sick and then recover b.) stay healthy, this is a non-virulent strain c.) are killed, this is the virulent strain Mice that are injected with only the S...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
4. Telomerases... a) are RNA-containing enzymes involved in replicating the ends of linear chromosomes b) cause aging in cells c) are structures located at the ends of eukaryotic chromosomes d) are needed by bacteria to complete synthesis of their chromosome ends e) are needed by bacteria to supercoil DNA 5. DNA methylation a) Modifies bases of tRNA molecules, allowing wobble b) Alters the activity of DNA polymerase by affecting access to the DNA c) Is a modification of histone tails...
QUESTION 5 Match these terms to the descriptions below. b. , is added onto mRNA during processing d , proteins that help initiate RNA synthesis a. spliceosome b. cap c. telomere d. transcription factors e. promoter a, removes introns from primary RNA transcript TATA-rich DNA sequence indicating the start site of a gene c repeating sequences found at the ends of linear DNA
Most questions answered within 3 hours.
-
Nitrogen at 500kPa and 150 degrees celsius in a rigid container
is cooled until its pressure...
asked 2 minutes ago -
Please I need help.
Java language
Method name: getScores
Return value is a String of numbers...
asked 13 minutes ago -
You purchased a bond at a price of $2,000. In 15 years when the
bond matures,...
asked 9 minutes ago -
what are the magnetic properties of the nitroprusside
{[Fe(CN)5(NO)]2-} anion?
asked 12 minutes ago -
Cash solvency is the government's long-run ability to pay all
the costs of doing business.
Question...
asked 18 minutes ago -
A cylindrical specimen of a metal alloy (1.48x10^1) mm in
diameter and (1.268x10^0) m long is...
asked 22 minutes ago -
let Z1, Z2, Z3 be iid ~N(0,1) .
Use moment generating function method to find
Y=2Z1+3Z2+6Z3-2
asked 25 minutes ago -
Problem 3: When Norway rats invade a new warehouse where living
conditions are ideal, they multiply...
asked 27 minutes ago -
If a company issues 10,000 shares of $8 par value common stock
at a market price...
asked 29 minutes ago -
(NURSE SURVEY) A researcher is investigating workplace concerns
among Nurses and Nurse assistants. They decide to...
asked 1 hour ago -
True or False
1.Bonds are sold at face value when the contract rate is equal
to...
asked 34 minutes ago -
1) A manufacturer knows that their items have a normally
distributed length, with a mean of...
asked 40 minutes ago