Answer:
No, we would not use a slant culture as a source of material for preparing a wet mount or for the preparation of a sample for flagella staining, because for this preparation a fresh culture is required to be used and slant cultures are stored for days which isn't fresh and therefore, the bacteria would go in a dormant stage and the mobility would become less or get completely absent.
2 Would you use a slant culture as a source of material for preparing a wet...
(a)You isolate a new pathogenic bacteria, from the sputum of a human patient, which you hypothesize was the cause of a severe respiratory infection. How could you use Koch’s postulates to prove your hypothesis? Which postulate would not be satisfied if the organism was a human-only pathogen? (b)What steps would you take to obtain a pure culture of the bacteria? What staining methods would you use to visualize the sample if you knew that the bacteria had a flagella? (c)...
: If your pipette was wet when you started to use it, how would your results be affected, if: a) it was wet with distilled water; b) it was wet with NaOH; c) it was wet with 1 M HCl; d) it was wet with your 0.1 M HCl
1. how do you determine the culture amount needed to prepare a bacterial smear? 2. what would be possible outcomes of a staining done in a slide with high cell concentrations? 3. what would be he problem if your smear has a low cell concentration?
You want to use Matrigel for cell culture with fibroblasts and endothelial cells (i.e., the cells would attach to it). Based on the source and composition of this material, how do you expect the cells to respond? Would the response vary for different cell types?
(1) Discuss YOUR perspective of the American culture. (2) Do YOU believe that our society identifies more with material culture or non-material culture? Explain why and give TWO of your own examples to support each of the positions.
After locating primary source material what criteria do you use when selecting information for inclusion in your introduction? Give an example of the material that should be included and the reason why? Provide an example of the material that should not be included and the reason why?
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
1. How does true motility differ from Brownian movement? 2. What could happen if you would touch the bottom of the motility agar with your needle during inoculation? 3 Explain why the following steps are essential during subculturing: a) Flaming the loop or needle prior to and after each inoculation. b) Cooling the loop or needle prior to obtaining the microorganism c) Holding the test tube caps in the hand as opposed placing them on the bench top. d) Flaming...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
You are preparing to write a four-source essay, a six-source essay, and a multiple-source essay. This exercise is designed to help you develop the skill of citing one source in a paragraph. Please examine the following short paragraph on a hypothetical paper about cybersecurity and the Internet of Things. The writer has made some errors in his integration of Shindell as a source. Please identify at least two errors. Describe what the errors are in a sentence or two. (You...