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45. Bioluminescence microscopy can be used for which reporter construct? a. lacz b. gfp c. lux d. All of the above e. None of the above The correct answer is C but please say why is it the correct answer?
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The correct answer is C: lux

lux (luminescence) codes for bacterial luciferase(Latin lucifer= lightbringer) which is a  class of oxidative enzymes that produce bioluminescence.There is no external requirement of light source to view it.

lacZ gene is a gene present in E. coli that encodes the protein beta-galactosidase and can emit luminescence only when fused with lux gene.

gfp exhibits bright green fluorescence only when exposed to light in the blue to ultraviolet range.

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