please answer 8 and 9 thank youHomework Answers
Question 1
The most critical step of gram staining is decolorizing. The decolorizing step should not last more than 15 seconds because if the decolorizer is left for a long time then even the gram-positive cells will lose the primary stain (crystal violet) and appear pink. Thus any inaccuracy in this step can lead to false results.
Question 2
Usually, ethyl alcohol and acetone are used as a decolorizer. Acetone and alcohol treatment dehydrates the peptidoglycan layer of the cell membrane that leads to cell shrinkage and tightening. The tight cell membrane unable the penetrance of crystal violet-iodine into the cell membrane.
Therefore the primary stain gets trapped in the cell wall of gram-positive bacteria giving it a purple-colored appearance. In gram, staining water is used only for rinsing and removing extra dye and decolorizer.
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please answer 8 and 9 thank you
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NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
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Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
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Please use the first three pictures to help me answer 3,4,5 thank u Text Direction に]...
Please
use the first three pictures to help me answer 3,4,5 thank u
Text Direction に] Align Text Convert to SmatArtArange Styl Paragraph Drawing The Best Medicine? As a prelude to nursing school, you've been volunteering with Dr. Rousseau this semester to pick up valuable experience. She's just finished the examination of two patients who complains of tightness in the chest, extreme difficulty in breathing, a deep cough, and a fever-she tells you these are all classic symptoms of pneumonia....
Microbiology ONLY answer 1 2 3 4 5 6 7 8 9 10 11 12 13...
Microbiology
ONLY answer
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3
4
5
6
7
8
9
10
11
12
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Salmonella is associated with the handling of contaminated beef True False Escherichia coli is a red-pigmented organism. True O False The results of the MR-VP test can be directly read from the test without the addition of any other chemicals. True False Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and...
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A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
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Please answer question 2. Thank you!
Case Study Questions 1. Cocaine (in the form of cocaine hydrochloride) is usually consumed by drinking, snorting, or injecting, but not by smoking. However, the free base form of cocaine is smoked. What is the likely reason for this? 2. What chemical reaction(s) would occur if NaOH were used instead of baking soda to make crack cocaine? Write a mechanism for the reaction(s) proposed. 3. Locate and read the article that Professor Martinez referred...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
Case Study #1 (1 of 3) V.Q., a 20-year old woman with no denies any previous history of Urinary Tract Infection, complains of burning on urination, frequent urination of a small amount, and bladder pain. She has no fever or pelvic area tenderness. A clean-catch midstream urine sample shows Gram negative rods on Gram stain. A culture and sensitivity test are ordered. Our patient is presumed to have a lower UTI. Question #1 What are some goals of the treatment...
Please
use the first three pictures to help me answer 3,4,5 thank u
Text Direction に] Align Text Convert to SmatArtArange Styl Paragraph Drawing The Best Medicine? As a prelude to nursing school, you've been volunteering with Dr. Rousseau this semester to pick up valuable experience. She's just finished the examination of two patients who complains of tightness in the chest, extreme difficulty in breathing, a deep cough, and a fever-she tells you these are all classic symptoms of pneumonia....
Microbiology
ONLY answer
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Salmonella is associated with the handling of contaminated beef True False Escherichia coli is a red-pigmented organism. True O False The results of the MR-VP test can be directly read from the test without the addition of any other chemicals. True False Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
Please answer question 2. Thank you!
Case Study Questions 1. Cocaine (in the form of cocaine hydrochloride) is usually consumed by drinking, snorting, or injecting, but not by smoking. However, the free base form of cocaine is smoked. What is the likely reason for this? 2. What chemical reaction(s) would occur if NaOH were used instead of baking soda to make crack cocaine? Write a mechanism for the reaction(s) proposed. 3. Locate and read the article that Professor Martinez referred...
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