Malate dehydrogenase catalyses the reaction L-malate + NAD+ <==> oxaloacetate + NADH + H+ and the following results were obtained, calculate Km and Vmax?
Malate dehydrogenase catalyses the reaction L-malate + NAD+ <==> oxaloacetate + NADH + H+ and the following results were obtained, calculate Km and Vmax?
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Malate dehydrogenase catalyses the reaction L-malate + NAD+ <==> oxaloacetate + NADH + H+ and the following results were obtained, calculate Km and Vmax?
The reaction catalyzed by malate dehydrogenase, Malate + NAD+ → oxaloacetate + NADH + H+ has...
The reaction catalyzed by malate dehydrogenase, Malate + NAD+ → oxaloacetate + NADH + H+ has a ΔG°′ value of +29.7 kJ · mol−1. (a) Would this reaction occur spontaneously in a cell? (b) How does the citrate synthase reaction (described in Problem 7) promote the malate dehydrogenase reac- tion in the cell? What is the overall change in free energy for the two reactions? 7. Citrate synthase catalyzes the reaction Oxaloacetate + acetyl-CoA → citrate + HS-CoA The standard...
Calculate AE for the following reaction; Oxaloacetate + NADH Malate + NAD+ E'' (Oxaloacetate /malate) =...
Calculate AE for the following reaction; Oxaloacetate + NADH Malate + NAD+ E'' (Oxaloacetate /malate) = -0.15V 2e ; E (NAD+/NADH) = -0.32V 2e +0.17V -0.32V -0.477 +0.47V -0.17V
In the citric acid cycle, malate is dehydrogenated to oxaloacetate in a highly endergonic reaction with...
In the citric acid cycle, malate is dehydrogenated to oxaloacetate in a highly endergonic reaction with a ΔG’o of +30 kJ mol-1: L‐malate + NAD+ ⇌ oxaloacetate + NADH + H+ A. Calculate the equilibrium constant K’eq of this reaction. What is the implication of this result? B. Let us suppose that you have gathered two sets of data concerning the malate dehydrogenase reaction: (a) From one set of data obtained from three different model systems (rat, mouse and pig)...
For the reaction: L-Malate + NAD⁺ ← → Oxaloacetate + NADH ΔE' = -0.154 n=2 T=298...
For the reaction: L-Malate + NAD⁺ ← → Oxaloacetate + NADH
ΔE' = -0.154
n=2
T=298 K
using the equation
ln (Keq) = (nFΔE'৹)/(RT)
Find Keq?
I know this sounds just plug and chug but am confused about the
signs of the numbers when plugged in. Please only answer if you're
of what you're doing!
Standard Reduction Potential of Some Biologically Important Half-Reactions, at pH 7.0 and 25°C (298 K) Half-reaction E' (V) 2H+ + 2e — H, (at standard...
QUESTION 8 Consider the reaction: Malate + NAD → Oxaloacetate + NADH (AG'º = +29.7 kJ/mol)...
QUESTION 8 Consider the reaction: Malate + NAD → Oxaloacetate + NADH (AG'º = +29.7 kJ/mol) A cell at 25°C has the following concentrations of these compounds: malate = 0.5 mm oxaloacetate=0.00002 mM • NAD = 1 mM NADH = 0.04 mm . Is the reaction spontaneous? QUESTION 9 Consider the reaction: 1,3-Bisphosphoglycerate (1,3-BPG) + ADP→ 3-Phosphoglycerate (3-PG) + ATP (AG' = -18.9 kJ/mol) A cell at 25°C has the following concentrations of these compounds: 1,3-BPG = 0.003 Mm ADP...
QUESTION THREE (20 MARKS) The enzyme malate dehydrogenase (MDIH) catalyses the following reaction: Malate +NADoxaloa...
QUESTION THREE (20 MARKS) The enzyme malate dehydrogenase (MDIH) catalyses the following reaction: Malate +NADoxaloacetate + NADH a) Which of the six major groups of enzymes (oxidoreductases, transferases, hydrolases s] l lyases, isomerases and ligases) does MDH belong to? Explain your answer. 2 mark b) What equipment would you use to assay this reaction; what would you measure and why 14 Marks] c) Malate dehydrogenase from pig liver mitochondria has a K M of 0.3 mM malate. Sketch a graph...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K), AG'' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. (malate) = 1.33 mm [oxaloacetate] = 0.200 mm [NAD) - 440 mm [NADH) - 180 mM AG: 36.9 KJ-mol-
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K). AG' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. [malate) = 1.43 mm [oxaloacetate) = 0.150 mM [NAD+] = 220 mm [NADH] = 88 mm kJ.mol-1 AG:
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the...
Consider the malate dehydrogenase reaction from the citric acid
cycle. Given the listed concentrations, calculate the free energy
change for this reaction at energy change for this reaction at 37.0
∘ C (310 K). Δ G ∘ ′ for the reaction is + 29.7 kJ/mol . Assume
that the reaction occurs at pH 7.
thank you
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy...
11.Calculate the standard free energy change for the oxidation of malate by NAD+ under physiological conditions....
11.Calculate the standard free energy change for the oxidation of malate by NAD+ under physiological conditions. Given, (30 points) Malate «Oxaloacetate + 2H+ + 2e- Eo’ = 0.166 V NAD++ H+«2e-+ NADH Eo’ = - 0.315 V
Calculate AE for the following reaction; Oxaloacetate + NADH Malate + NAD+ E'' (Oxaloacetate /malate) = -0.15V 2e ; E (NAD+/NADH) = -0.32V 2e +0.17V -0.32V -0.477 +0.47V -0.17V
For the reaction: L-Malate + NAD⁺ ← → Oxaloacetate + NADH
ΔE' = -0.154
n=2
T=298 K
using the equation
ln (Keq) = (nFΔE'৹)/(RT)
Find Keq?
I know this sounds just plug and chug but am confused about the
signs of the numbers when plugged in. Please only answer if you're
of what you're doing!
Standard Reduction Potential of Some Biologically Important Half-Reactions, at pH 7.0 and 25°C (298 K) Half-reaction E' (V) 2H+ + 2e — H, (at standard...
QUESTION 8 Consider the reaction: Malate + NAD → Oxaloacetate + NADH (AG'º = +29.7 kJ/mol) A cell at 25°C has the following concentrations of these compounds: malate = 0.5 mm oxaloacetate=0.00002 mM • NAD = 1 mM NADH = 0.04 mm . Is the reaction spontaneous? QUESTION 9 Consider the reaction: 1,3-Bisphosphoglycerate (1,3-BPG) + ADP→ 3-Phosphoglycerate (3-PG) + ATP (AG' = -18.9 kJ/mol) A cell at 25°C has the following concentrations of these compounds: 1,3-BPG = 0.003 Mm ADP...
QUESTION THREE (20 MARKS) The enzyme malate dehydrogenase (MDIH) catalyses the following reaction: Malate +NADoxaloacetate + NADH a) Which of the six major groups of enzymes (oxidoreductases, transferases, hydrolases s] l lyases, isomerases and ligases) does MDH belong to? Explain your answer. 2 mark b) What equipment would you use to assay this reaction; what would you measure and why 14 Marks] c) Malate dehydrogenase from pig liver mitochondria has a K M of 0.3 mM malate. Sketch a graph...
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K), AG'' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. (malate) = 1.33 mm [oxaloacetate] = 0.200 mm [NAD) - 440 mm [NADH) - 180 mM AG: 36.9 KJ-mol-
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy change for this reaction at 37.0°C (310 K). AG' for the reaction is +29.7 kJ/mol. Assume that the reaction occurs at pH 7. [malate) = 1.43 mm [oxaloacetate) = 0.150 mM [NAD+] = 220 mm [NADH] = 88 mm kJ.mol-1 AG:
Consider the malate dehydrogenase reaction from the citric acid
cycle. Given the listed concentrations, calculate the free energy
change for this reaction at energy change for this reaction at 37.0
∘ C (310 K). Δ G ∘ ′ for the reaction is + 29.7 kJ/mol . Assume
that the reaction occurs at pH 7.
thank you
Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentrations, calculate the free energy change for this reaction at energy...
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