Question

The following is part of the original in vitro translation data obtained by Dr. Har Gobind Khorana:

Synthetic mRNA Polypeptide synthesized (AC), (i.e., ACACAC......) (Thr-His). (AUC), (lle-lle), and (Ser-Ser), and (His-His). (UCG)n (Ser-Ser)n and (Arg-Arg), and (Val-Val)n

Hint: Remember that there is often wobble in the third position of the codon.

A) What is/are the codons for Ile? _______

B) What is/are the codons for His? ____

C) What is/are the codons for Ser? ____

D) What is/are the codons for Thr? _____

Answer 1

different codons in the sequence are obtained, in each reading frame there are three reading frames, first reading frame starts with the first nucleotide, the second reading frame starts with the second nucleotide, and the third reading frame starts with the third nucleotide. In all three sequences in all the reading frames all the codons are same.

D ACACACACACACACAC codons are ACA .The CAC- His 2) AUCAUC AUC AUCAUC Auc codons are Auc UCA CAU Ser His 3) UCGUCGUCGUCGUCG codons are UCG - Sez CGW-Arg Guc Val.

a) codons for Ile is

AUC.

b) codons of Histidine are

CAU, CAC.

c) codons for serine are

UCA, UCG

d) Codons for Thr is ACA