During post transcriptional modification of eukaryotic pre-mRNA introns are removed and exons are rearranged in a specific order to generate a matured mRNA with required sequence by alternate splicing.
Number of nucleotides in mRNA = Sum of nucleotide length in (Exon 1 + Exon 2 + Exon 3 + Exon 4)
Number of nucleotides in mRNA = 219 + 54 + 162 + 45
Number of nucleotides in mRNA = 480
Since three nucleotides collectively (triplet codon) code for a single amino acid number of amino acids in the given protein = Number of nucleotides / 3 = 480 / 3 = 160 amino acids.
Molecular weight of one amino acid is about 110 Da then molecular weight of protein with 160 amino acids = 160 x 110 = 17,600 D = 17600/1000 KDa =17.6 KDa
A cukaryotic pre-mRNA transcript has 5 exoms and 4 introms of variable length, according to the...
Calculate the minimum length of time required for E. coli RNA polymerase to synthesize an mRNA that encodes a 100 kDa protein. The molecular weight of an amino acid is approximately 110 Da. E. coli RNA polymerase has a transcription rate of approximately 50 nucleotides per second. Round your answer to the nearest whole number.
QUESTION 7 Consider the hypothetical gene show here. The gene coding sequence consists of 4 exons (shown in solid colors). The 5' UTR and 3' UTR are shown as striped boxes. 25 150 200 300 400 25 Based on the lengths of the exons, you should be able to calculate a rough estimate of the protein encoded by this gene, assuming that all 4 exons are translated and the protein does not undergo any significant processing (cleavage or chemical modifications)....
1. (4 points) Given that the average molecular weight of an amino acid is 110 daltons (Da), determine the following. Remember to convert between daltons and kilodaltons (kDa) where necessary. Each of your answers must be numerically correct and expressed in proper units (1 pt) and show how the value was arrived at using the amino acid molecular weight above (1 pt) for you to receive full credit. A. A molecule of human a-tubulin has a molecular weight of 50.1...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Molecular Bio lab. HELP!!
Here is the first part: the sequence traces and the entire
sequence. i just need the last 3 tasks. i color coded the ends so
you can see where it overlaps and connects
In the files section for your group there is a simulated output from an automated DNA sequencer using a variation of the classic Sanger method. (If you want to print it, it is formatted for legal sized paper.) This sequence encodes a protein...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in process called DNA curs in the nucleus of s acest FS Parent strand Parent strand Newly replicated DNA Newly replicated DNA- SA0 Daughter DNA molecule Daughter DNA molecule Figure 8.2: Overview of DNA replication and illustration of complementary base pairing. DNA must replicate before cell division so that each new daughter cell receives an exact copy of the parent DNA. 1. Replication begins when...
please answer all pre-lab questions 1 through 5. THANK YOU!!!
this is the manual to give you some background.
the pre-lab questions..
the pre-lab sheet.
Lab Manual Lab 10: String Waves & Resonance Before the lab, read the theory in Sections 1-3 and answer questions on Pre-lab Submit your Pre-lab at the beginning of the lab. During the lab, read Section 4 and follow the procedure to do the experiment. You will record data sets, perform analyses, answer questions, and...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
need help with questions 5,6, and 7
Assignment #2 WO Chapter 3, 4, 5, 6 & 7: Energy Balance and Weight Management Directions: Answer the following questions using the formulas found in Chapter 6 & 7. All work must be added to receive full credit (10-points). By completing this assignment you will satisfy #4 ECO objective and #11 and #12 MO/CO. 1. Calculate the EER for a 25-year-old man who weighs 198 pounds, is 5'8" tall and has a low...