i need to make 250ml of a buffer containing: 15mMtris ph 7.6 200microM EDTA. my stock solutions are: 1.5M tris ph 7.6 500mM EDTA. how much of each do I need and how much water
i need to make 250ml of a buffer containing: 15mMtris ph 7.6 200microM EDTA. my stock...
You must make a 1x TAE running buffer (40mM tris (pH 7.6), 20mM acetic acid and 1mM EDTA) using tris base (FW= 121.14g/mol), glacial acetic acid (17.4M) and 0.5M EDTA. show how much of each compound you would add to make 1L.
During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml of the A150 buffer?
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
Question 2 1 pts You need to make a 500 mL buffer containing 35 mM NaCl and 50 mM Tris buffer. You already have a 5 M NaCl stock solution and a 1 M Tris stock solution. How much NaCl stock do you need (mL)?
Calculate how much of each solution (and water!) you would need to make 100 mLs of a 0.1 M Tris/0.05M EDTA solution from 1M Tris and 1 M EDTA stock solutions?
I need to make a 0.50M Tris buffer with a pH of 8.75. I have access to Tris (a weak acid) and the sodium salt of Tris (think A-). The Ka of Tris is 8.5x10-9 and the pKa of Tris is 8.07. What are the [HA] and [A-] concentrations of this 0.50M buffer at pH 8.75? Part B: [A-] = _____
3). I need to make a 0.50M Tris buffer with a pH of 8.75. I have access to Tris (a weak acid) and the sodium salt of Tris (think A-). The Ka of Tris is 8.5x10-9 and the pKa of Tris is 8.07. What are the [HA] and [A-] concentrations of this 0.50M buffer at pH 8.75? Part A: [HA] = _____
3). I need to make a 0.50M Tris buffer with a pH of 8.75. I have access to Tris (a weak acid) and the sodium salt of Tris (think A-). The Ka of Tris is 8.5x10-9 and the pKa of Tris is 8.07. What are the [HA] and [A-] concentrations of this 0.50M buffer at pH 8.75? Part B: [A-] = _____
I need help doing problem number 5 listed below. If someone could show the work required for this problem it would be a great help! Below is a list of solutions you will need for the mini-prep. Make 5mL of each solution as a team and store each in a 15mL Falcon tube. Store solution 1 and 70% ethanol at 4 degrees Celsius. Solution 3 and Elution Buffer can be stored at room temperature. Show your calculations in the space...
Show calculations for the solutions. Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...