You must make a 1x TAE running buffer (40mM tris (pH 7.6), 20mM acetic acid and 1mM EDTA) using tris base (FW= 121.14g/mol), glacial acetic acid (17.4M) and 0.5M EDTA. show how much of each compound you would add to make 1L.
You must make a 1x TAE running buffer (40mM tris (pH 7.6), 20mM acetic acid and...
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
1. Show your working out for the following calculation a) TENS buffer: Make from 0.1M NaOH from stock 10M 0.2% SDS from 10% stock 1mM EDTA from 0.5M stock 10mM Tris pH 7.5 from 1M stock How would you make 200ml of TENS buffer from these stock solutions? b) 5M LiCl How would you make 5M LiCl (MW=42.40g/mol)? c) 70% Ethanol How would you make 1L of 70% Ethanol? d) HisTag Protein Purification Solutions Charge Buffer 50mM NiSO4.6H2O Binding...
You are asked to make one litre of 0.2M Tris-CI buffer pH 8.8, using Tris base and IM hydrochloric acid. a) given the MW of Tris is 121.1, how much Tris would you weigh out to make one litre of b) what is the ratio of [Tris base] to [Tris acid] in the final buffer solution? (pKa of Tris is c) what is the total concentration of Tris (i.e. Tris base form+Tris acid form) in the final 0.2M Tris-Cl buffer?...
Show calculations for the solutions. Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
how do you make 500mL of TE buffer (20mM Tris-HCl, 0.75mM EDTA)
Which of the following compounds would make the best buffer at pH 7.5? Acetic acid, pKa= 4.76 N-tris(hydroxymethyl) methylglycine (Tricine), pKa = 8.15 N-2-hydroxyethylpiperazine-N’-ethansulfonic acid, pKa2= 7.6 Glycine, pKa2= 9.9 Tris(hydroxymethyl)aminomethane (Tris), pKa = 8.3 I believe 3 is the correct answer and therefore I am trying to answer the below question with this information. Using the answer that you selected from question 1 above: a. Explain how you would make the buffer starting with 1M HA and 1M A-...
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
i need to make 250ml of a buffer containing: 15mMtris ph 7.6 200microM EDTA. my stock solutions are: 1.5M tris ph 7.6 500mM EDTA. how much of each do I need and how much water
To a 25mL cylinder add 1.25mL of a 2M stock solution of Trizma base. Calculate the concentration of the Tris-base in your cylinder if the final volume is filled with 25ml water. ( 1 mark)Calculate the ratio of Tris- base to Tris-acid required, using the Henderson- Hasselbach equation. (2 marks) You are provided with a 1M Tris- HCL solution. Using a Tris buffer concentration of 100mM and ratios from step 2 above, calculate the concentration of Tris HCl required and...
9,10,12 petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...