9) you are required to prepare 1000ml of 1X Tris-borate-EDTA (TBE) gel running buffer from 10X TBE concentrate. How much of the stock buffer is required? How much of the solvent is required?
9) you are required to prepare 1000ml of 1X Tris-borate-EDTA (TBE) gel running buffer from 10X...
TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to 1X for use. The 5X stock consits of 250mM Tris base, 250mM Boric Acid and 2.5mM EDTA. Calculate the mass of EDTA (mw=372.24) required for 500mL of the 5X stock. please explain in detail, thanks
a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.
You must make a 1x TAE running buffer (40mM tris (pH 7.6), 20mM acetic acid and 1mM EDTA) using tris base (FW= 121.14g/mol), glacial acetic acid (17.4M) and 0.5M EDTA. show how much of each compound you would add to make 1L.
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid
k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA, 1mM Tris) if given NaCl solid (gram molecular weight = 58.44), a 5M stock EDTA, and a 100mm stock Tris?
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
Describe how much agarose powder (g), 10X TBE (ml), water (ml) and 20,000X greenglo (ul) you would need to prepare a 4.3% 50ml agarose gel.
how do you make 500ml of 1X SDS running buffer from a 5X stock solution?