1. Show your working out for the following calculation
a)
TENS buffer: Make from
0.1M NaOH from stock 10M
0.2% SDS from 10% stock
1mM EDTA from 0.5M stock
10mM Tris pH 7.5 from 1M stock
How would you make 200ml of TENS buffer from these stock solutions?
b)
5M LiCl
How would you make 5M LiCl (MW=42.40g/mol)?
c)
70% Ethanol
How would you make 1L of 70% Ethanol?
d)
HisTag Protein Purification Solutions
Charge Buffer
50mM NiSO4.6H2O
Binding Buffer
5.0mM Imidazole (MW=68.08g/mol)
0.5M NaCl (MW=58g/mol)
20mM Tris pH8
Wash Buffer
60mM Imidazole
0.5M NaCl
20mM Tris pH8
Elution Buffer
1.0M Imidazole
0.5M NaCl
20mM Tris pH8
How would you make 50ml of the Wash Buffer? (assume you have a solution of 1M Tris pH 8)
TENS Buffer contains Tris, EDTA and NaOH.
1 Litre of TENS contains
10ml of 10 mM Tris-HCl
2ml of 1 mM EDTA
So for 200 ml you will require 2ml of 1M Tris-Cl pH 8 and 0.4 ml of 1 mM EDTA to be dissolved in 197.6 ml of water.
Adjust the pH of the solution using NaOH solution
1. Show your working out for the following calculation a) TENS buffer:  
You must make a 1x TAE running buffer (40mM tris (pH 7.6), 20mM acetic acid and 1mM EDTA) using tris base (FW= 121.14g/mol), glacial acetic acid (17.4M) and 0.5M EDTA. show how much of each compound you would add to make 1L.
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