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Q3. Bilirubin is a product of the catabolism of heme, and it is the compound that...

Q3. Bilirubin is a product of the catabolism of heme, and it is the compound that gives urine its yellow color.

a. What kind of plot is this?

b. How is NAD+ used (what is its role) in this experiment?

c. What does this plot show about the interaction of bilirubin with isocitrate dehydrogenase? (5

words or less.)

Q3. Bilirubin is a product of the catabolism of he

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Answer #1

(a) The graph is Lineweaver-Burk plot.
(b) NAD+ is ued as Substrate in this experiment
(c) Bilirubin is acting as a competitive inhibitor as it is competing with the substrate for the active site of isocitrate dehydrogenase enzyme. By seeing the plot, we can easily tell to which category the inhibitor belongs to.

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