How do I calculate the approximate melting/annealing temperature of a primer containing this sequence (which anneals...
How do I calculate the approximate melting/annealing temperature of a primer containing this sequence (which anneals perfectly to its target): 5' GAT TTA CCG TAC TGG GCA ACG 3' More importantly than the actual Tm, I would like to understand HOW to get to there. Thanks!!!
Homework Answers
At high temperature, the DNA segment would be denatured, and it could be annealed to short primers at a lower temperature. A DNA polymerase could be added, along with nucleotide substrates (dATP, dTTP, dCTP, and dGTP), to cause synthesis from the 3' end of each primer, using the annealed DNA as template.
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How do I calculate the approximate melting/annealing temperature
of a primer containing this sequence (which anneals...
Adenosine deaminases modify adenosines to form _____________________, which is then read as _________________________ by the translation...
Adenosine deaminases modify adenosines to form
_____________________, which is then read as
_________________________ by the translation and splicing systems,
as well as by reverse transcriptase.
Cephalopods like squid and octopus use this form of editing very
extensively in their protein-coding regions. For each of
the following, please indicate how these enzymes can alter the mRNA
to produce the indicated codon changes.
I (Ileu) is changed to V (val):
K (Lys) is changed to E (Glu):
T (Thr) is changed to A (ala):...
Adenosine deaminases modify adenosines to form
_____________________, which is then read as
_________________________ by the translation and splicing systems,
as well as by reverse transcriptase.
Cephalopods like squid and octopus use this form of editing very
extensively in their protein-coding regions. For each of
the following, please indicate how these enzymes can alter the mRNA
to produce the indicated codon changes.
I (Ileu) is changed to V (val):
K (Lys) is changed to E (Glu):
T (Thr) is changed to A (ala):...
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