Question

13. Protein B regulates ubiquitination of the protein encoded by gene A If the level of protein B changes, what else do we expect to change (please answer yes/no for each)? Transcription of gene A? Splicing of gene A? Rate of translation of RNA copies of gene A? Overall level of protein A in the cell? yes 14. Imagine that a protein regulates a gene. Given that it regulates the other gene at the following levels, circle all that will be affected for the regulated gene. Transcription pre-RNA RNA rate-of-translation protein-level Ubiquitination pre-RNA RNA rate-of-translation protein level Translation Splicing pre-RNA RNA rate-of-translation protein level pre-RNA RNA rate-of-translation protein level

Answer 1

13.Ubiquitination is a process of adding ubiquitin groups on any proteins destined to proteasomal degradation.

This process occurs with few proteins which has completed their task in the cell or damaged or unfolded.

So this process has no relation with transcription and translation of any gene but it can change the overall protein content inside cell.

So No for transcription of gene A

No for splicing of gene A

No for translation of RNA copies of gene A

Yes for overall protein A in the cell.

14.a.If any protein affect the transcription of a gene then it will affect the formation of pre mRNA.Further processes like splicing(which result in formation of mature mRNA),translation will not be affected by this protein.

b.If any protein affect ubiquitination then overall protein level will be affected as we discussed in question no 13.

c.at translation level regulation,rate of translation and protein level will be affected.

d. If it regulates splicing then only formation of mature RNA will be affected.