what is the antibiotic resistance marker for pBluescript for cloning? Is it Ampicillin or is it...
what is the antibiotic resistance marker for pBluescript for cloning?
Is it Ampicillin or is it B-galactosidase?
Homework Answers
I believe the correct answer to be:
Option A) Ampicillin.
As B galactosidase is not an antibiotic marker, it does not provide any kind of resistance to the cell. It simply gives the colony a blue colour in presence of X-Gal and is used as a marker in transformation, not cloning.
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what is the antibiotic resistance marker for pBluescript for
cloning?
Is it Ampicillin or is it...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning...
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
(a) How is the activity of beta-galactosidase useful in bacterial gene cloning? (b) How does this...
(a) How is the activity of beta-galactosidase useful in bacterial gene cloning? (b) How does this use for beta-galactosidase differ from drug selection using antibiotics like ampicillin? Give at least a 2-sentence answer for each part.
help asap please The purpose of including an ampicillin resistance gene in a plasmid into which...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
The veterinarian asks Karen to reconstitute a bottle of the antibiotic Ampicillin to administer intravenously. The...
The veterinarian asks Karen to reconstitute a bottle of the antibiotic Ampicillin to administer intravenously. The dosage of the drug is 3 mg/lb. Ampicillin comes in 3 g vials. Each vial needs to be mixed with a diluent for a final concentration of 300 mg/ml. Patient weighs 690kg. What dose weight of Ampicillin should Winnie receive? (2 points) What dose volume of Ampicillin should Winnie receive? (2 points) How many milliliters of diluent should be mixed with each 3 g...
What is the danger of using an ampicillin resistance gene as part of a bacterial transformation?...
What is the danger of using an ampicillin resistance gene as part of a bacterial transformation? What could happen if this gene was spread to wild bacterial population? What are four ways the ampicillin resistance genes could be prevented from escaping to the wild. Briefly explain each method of prevention.
What is antibiotic resistance? Thoroughly explain. What is pesticide resistance? Thoroughly explain. What are the causes...
What is antibiotic resistance? Thoroughly explain. What is pesticide resistance? Thoroughly explain. What are the causes of antibiotic and pesticide reistances? Thoroughly discuss. What can humans do to prevent development and expansion of antibiotic and pesticide resistances? Thoroughly justify.
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you...
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you try to deactivate it by transformation. What would be a selective medium to check if this deactivation was well done? 1. Rich medium + ampicillin 2. Rich medium - ampicillin B) Considering question A and if the deactivation went well, in which case (s) will the transformed bacterial cells be alive? 1. Rich medium + ampicillin 2- Rich medium - ampicillin
What part of the pGlo plasmid is inducible a) the genes for ampicillin resistance b) the...
What part of the pGlo plasmid is inducible a) the genes for ampicillin resistance b) the genes to break down arabinose c) the genes to form a pilus d) all the genes are inducible
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. This enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial colony transformed with...
help asap please
The purpose of including an ampicillin resistance gene in a plasmid into which you are cloning a piece of DNA is: This is used to prevent expression of other ampicillin resistance genes in the bacterial genome. This enables the creation of ampicillin-resistant bacteria for biomedical research. ChmThis enables selecting a bacteria colony transformed with a plasmid with a DNA insert ligated in as a means of amplifying a specified piece of DNA. This enables selecting a bacterial...
A) You are interested in a gene coding for ampicillin resistance at a bacteria and you try to deactivate it by transformation. What would be a selective medium to check if this deactivation was well done? 1. Rich medium + ampicillin 2. Rich medium - ampicillin B) Considering question A and if the deactivation went well, in which case (s) will the transformed bacterial cells be alive? 1. Rich medium + ampicillin 2- Rich medium - ampicillin
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