enzymes are biocatalyst which enhance rate of reaction with out taking active part in main reaction.
enzymes catalyze reaction in our body and carry out in very lower energy. enzymes decrease activation energy of reaction. if enzymes are not present in body then it will take too much time and energy to carry out daily metabolic process. in body as wel as enzymes are used in daily life also like in detergents, food processing, medicines etc to gives better results.
enzymes are mode up of complex structure of protein and in proteins are stable then enzymes will gives better response.
so that for optimum rate of reaction enzymes need optimum pH, temperature, as well as concentration of inorganic cofactor, ions etc. which stabilize protein structure of enzyme.
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
What is the biochemical basis for enzymes exhibiting pH optimum?
COST-LAB QUESTIONS: 1. What happens to enzyme activity as the enzyme concentration is increased? 2. Based on your results, what is the optimum pH for lactase? 3. Do all enzymes in the body have the same optimum pH? Why or why not? 4. What happens to enzyme activity as the substrate concentration is increased? Explain why this occurs.
Why most enzymes have an optimum pH of around 7 while pepsin will be optimum at ph 2? Give a detail explanation!
Enzymes as diagnostic tools. How do enzymes help in the diagnosis of human disease? Why are enzyme tests only the first step in the diagnosis of disease?
When running enzyme assays, why do you need to normalize enzyme activity to total protein concentration? What does the specific activity mean in the research lab where enzymes are used? (Hint: are enzymes always pure?)
Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme, water and buffer be added to the microcentrifuge tube for a restriction digest? If lambda DNA is linear, how many times would the enzyme have to cut the DNA to generate five DNA fragments? Would a shorter DNA fragment move faster or slower through the agarose gel than a longer fragment? Why?
Which statement characterizes an enzyme catalyzed reaction? Enzymes change the reaction equilibrium Enzyme-substrate binding is irreversible Enzymes do not increase reaction rates Enzymes increase reaction rates Enzymes defy the space-time continuum
yule grapn below comparing two different enzymes with different enzyme kinetics. a. Do the graphs have any of the same values for their Vmax, Vmax/2 or Km? If so which are the affinity for substrate same which are different? Enzyme with high Enzyme with low affinity for substrate Difference in reaction rate at low [5] Reaction rater Low (S] Substrate concentration [5] b. READ THIS: Enzyme-substrate affinity is the tendency for enzyme and substrate to bind together and form the...
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c