You have a 5 mg/ml sample of GST (26 kDa). What is GST’s concentration in micromolar?
You have a 5 mg/ml sample of GST (26 kDa). What is GST’s concentration in micromolar?
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A high concentration is greater than 1 mg/ml and low concentrations are below 0.1 mg/ml (roughly). If you obtained a sample of MDH that was 10 micromoles/L, what is the concentration in mg/ml? Assume that the molecular weight for MDH is 32 kDa. 2. Using Beer's Law, determine the concentration of your sample if the UV-vis reading at 280 nm was 1.2 absorbance units and...
if a protien of 19.6 kda has a conentration if 0.2mg/ml. what is the concentration if the protien in micro Molar
Twenty-four hour urine sample: Total volume = 1350 ml Inulin concentration = 1.86 mg/ml, Creatinine concentration = 3.6 mg/ml Sodium concentration = 0.17 mEq/ml, Urea concentration = 24.0 mg/ml Potassium concentration = 0.12 mEq/ml, PAH concentration = 10 mg/ml Plasma sample taken at the midpoint during urine collection: Hematocrit = 43% Inulin concentration = 0.013 mg/ml, Creatinine concentration = 0.019 mg/ml Sodium concentration = 0.13 mEq/ml, Urea concentration = 0.28 mg/ml Potassium concentration = 0.008 mEq/ml, PAH concentration = 0.014...
You have a stock of streptomycin (antibiotic) with a concentration of 200 mg/mL. You add water until the concentration of streptomycin is 8 mg/mL. What dilution have you performed? (Hint: Think of how many times less concentrated your final concentration is compared to your initial. Using C1V1=C2V2) This is all the information provided in the question.
You have an analyte with a K(h,w) of 5890 in an aqueous sample with a volume of 100.0 mL. You extract it with 20.0 mL hexane and determine its concentration in the hexane layer to be 75 micromolar. Solve for its concentration in the original aqueous phase.
You have a vial of Penicillin with a concentration of 10 mg per ml. You are asked to administer 2ml of 1:100 dilution of the drug to your patient. How much of the drug is your patient getting? Answer is either in mg or microgram Those are the only possible answers given.
29. Complete the problem on the slide titled 'Sample Problem". If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg/mL) of a solution of this protein which has an absorbance (A) =0.2 at 2 = 280 nm? How many grams of protein are present in 1 mL of this solution? Protan G:B1 3 tyrosine & One tryptophan
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...