if you performed isoelectric focusing on a series of amino acids, at which pH would arginine...

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if you performed isoelectric focusing on a series of amino acids, at which pH would arginine...

if you performed isoelectric focusing on a series of amino acids, at which pH would arginine stop migrating?

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Answer 1

Answer #1

Isoelectric point is the pH at which molecule carry no charge that is it is neutral. For Amino acid, pI is average of pKa of groups after and before neutral form. When Amino acid is neutral, it will not migrate towards respective electrode.

Arginine contain basic side chain, thus it's pI is basic.

pH is 10.76

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if you performed isoelectric focusing on a series of amino acids, at which pH would arginine...

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