Question

Ovalbumin is the major protein component of egg whites. The chicken ovalbumin gene contains eight exons and seven introns. Would amplification of the chicken gene from isolated chicken chromosomes be an appropriate starting point to create a plasmid for recombinant expression of ovalbumin in E. coli? Justify your answer with as much detail as you are able to

Answer 1

• If the gene is amplified from the chromosome of chicken, it would contain exon as well as intron parts in-between the gene. if you clone this fragment into a plasmid and transform into E.coli, it will not be expressed (ovalbumin protein will not be made in the E.coli cell) as E.coli(all eubacteria)genes contain only exons or coding region, they don't have such intron system thus they don't have the splicing mechanism of mRNA so as there is presence of introns in the gene, it will not expressed in the E.coli cell