S. marcescens is positive for esculinase activity on BEA agar. How would you need to change...
S. marcescens is positive for esculinase activity on BEA agar. How would you need to change the protocol to find mutations in genes required for this activity, rather than mutations in pigment production genes ?
Homework Answers
As the previous protocol being used is not given, we can assume that using ferric chloride in the media will help in detecting if the genes for esculinase activity are mutated or not.
If the genes are not mutated, then esculinase activity will take place to hydrolyze Esculin into esculetin and dextrose. The esculetin will react with ferric chloride and form a black brown colour. So, if the black brown colour is not formed, then we can suggest that the genes for esculinase activity are mutated.
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S. marcescens is positive for esculinase activity on BEA agar.
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