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At a minimum, how many DnaE molecules are replicating the E. coli chromosome during log phase?
At a minimum, 2 (option b) DnaE molecules are replicating the E. coli chromosome during the log phase.
At a minimum, how many DnaE molecules are replicating the E. coli chromosome during log phase?...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
The E. coli chromosome contains an operon with the following components: an operator, a promoter, and 4 structural genes identified as A,B,C, and D. Diagram this operon in the following manners: A. Inducible, positive regulation. B. Repressible, negative regulation.
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
10 points. The thr and leu operons are 55% linked in the E. coli chromosome. A P1 lysate from a thr-leu+ strain is used to transduce a thr+ leu- strain to leu+ using a minimal glucose plate. What possible recombinants can be obtained (specify them as thr+/- leu +/-)? 10 points. An E. coli strain with a Tn10 that is 75% linked to a leu A mutant allele (Leu-) is used to transduce wt E. coli to TetR on rich...
When measuring the growth curve of E. coli by spectrophotometer, the death phase is not normally seen as a change in absorbance because a. the cells are dividing most rapidly at that stage b. the cells are still “gearing up” to start dividing before log phase c. the spectrophotometer inhibits the death phase, so cells stay in stationary phase d. dead cells absorb and diffuse light to nearly the same degree as live cells do e. live cells are black,...
How many molecules of acetyl CoA will be formed during β-oxidation of a fatty acid containing a chain of ten carbon atoms? a. 2 b. 3 c. 4 d. 5 e. 10
A diploid organism has a chromosome number 2n=6. How many chromatids will be present during metaphase of meiosis? Select one: O a. 3 b. 9 O c. 24 Od. 12 e. 6
During the log phase of growth a. bacteria are not multiplying b. bacteria are dying, resting and multiplying c. bacteria are just multiplying but slower than in log phase d. bacteria are multiplying rapidly
the drosophila chromosome #3 is 27.9 million base pairs long. 1. how many nucleosomes and histones would be present on one of these chromosomes at the mitotic metaphase plate? 2. if this chromosome is 30% G/C, how many hydrogen bonds and phosphodiester linkages would a single chromosome have before the S phase? 3. how many of these G0 chromosomes would you need to stretch end to end to equal one kilometer?
4. Telomerases... a) are RNA-containing enzymes involved in replicating the ends of linear chromosomes b) cause aging in cells c) are structures located at the ends of eukaryotic chromosomes d) are needed by bacteria to complete synthesis of their chromosome ends e) are needed by bacteria to supercoil DNA 5. DNA methylation a) Modifies bases of tRNA molecules, allowing wobble b) Alters the activity of DNA polymerase by affecting access to the DNA c) Is a modification of histone tails...