you isolate a virus and discover that it contains a single-stranded (ss) RNA genome. How would...
you isolate a virus and discover that it contains a single-stranded (ss) RNA genome. How would you determine if the virus has a + sense of a - sense ssRNA genome?
Homework Answers
By sequencing of RNA extracted from isolated virus
- If the isolated RNA contains the cis functional elementary sequences like UTRs, shine dalgarno sequences, star codon, stop codon etc. in preferred direction on that isolated strand then that would be the (+) sense ssRNA
- If those sequences are present as complementary sequence from the sequence of isolated RNA then that would be the (-) sense ssRNA
Because (+) sense RNA can be translated but for (-) sense RNA, it has to be converted into (+) sense RNA, then it will be translated.
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