You harvested macrophages and resuspended them in 1 ml of PBS. You then took a small...

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You harvested macrophages and resuspended them in 1 ml of PBS. You then took a small...

You harvested macrophages and resuspended them in 1 ml of PBS. You then took a small aliquot (1 microliter) and made a 1/1000 dilution for counting. The count revealed that there were 1 x 10e3 cells/ml in the diluted sample. Calculate the concentration of cells in the original cell suspension.

Note: 10e3 (e=exponential). This program does not display exponentials correctly)

1 x 10E7

1x10E6

1x10E8

1x10E9

science biology

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Answer 1

Answer #1

Answer: Option B is correct
Explanation:
Dilution = 1 uL in 1000 uL = 1:1000 diltion = 10^-3 dilution
Concentration of the diluted sample = 1 X 10^3 cells/mL
Concentration of the undiluted sample = 1 X 10^3 X 10^3 cells/mL
= 1 X 10^6 cells/mL

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Answer 2

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