Question

1) Select the cellular (and subcellular, if relevant) location for each enzyme listed below. (Cytoplasm,Inner membrane of mitochondria, outer membrane of mitochondria, Matrix of mitochondria)

Phosphofructokinase:

ATP synthase:

Hexokinase:

Pyruvate dehydrogenase:

Aldolase:

Citrate synthase:

NADH dehydrogenase complex:

2) Based on our discussion in class, which enzyme performs the first committed step in glycolysis? ( i picked hexokinase on this and got it wrong)

a. phosphofructokinase

b. pyruvate kinase

c. hexokinase

d. cytochrome

e. aldolase

Answer 1

Answer:

1)

Phosphofructokinase----Cytoplasm (glycolysis)

ATP synthase ---Inner membrane of mitochondria (electron transport chain)

Hexokinase----Cytoplasm (glycolysis)

Pyruvate dehydrogenase----Matrix of mitochondria (pyruvate oxidation)

Aldolase----Cytoplasm (glycolysis)

Citrate synthase----Matric of mitochondria (critic acid cycle)

2). C. Hexokinase

Explanation:

a. phosphofructokinase----3^rd enzyme

b. pyruvate kinase---10^th enzyme

c. hexokinase---1^st enzyme

d. cytochrome---not involved in glycolysis

e. aldolase---4^th enzyme