why you will start by filtering the 1 ml dilution of river water and working your...
why you will start by filtering the 1 ml dilution of river water and working your way up to the 50 ml dilution and not the other way around (in membrane filter technique)
Homework Answers
Answer :)
From many experiments, dilution of 1 ml river water to 50 ml dilution, makes the water clears from the coliform and other harmful bacteria, mostly. Therefore, in the membrane filter technique, 1:50 dilution is preferred. In some countries, 1:100 dilution is preferred where excessive contamination is present.
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why you will start by filtering the 1 ml dilution of river water
and working your...
4. While filtering your product on the Buchner funnel, you washed the crystals with 15 mL of cold...
4. While filtering your product on the Buchner funnel, you washed the crystals with 15 mL of cold water. What is the purpose of washing the crystals? a. I alss hale b. Why do you think it was necessary for the water to bè cold Ttis neressam For the w becavse itiatw melt thestals Instructor Initials_ CHL 161 Experiment #8 (Rev. 1/7/2019)
4. While filtering your product on the Buchner funnel, you washed the crystals with 15 mL of cold...
Suppose you were to make your own standards (via serial dilution) using 6 tubes. You start...
Suppose you were to make your own standards (via serial dilution) using 6 tubes. You start with tube #1- you have 450 uL of assay buffer and add 50 uL of estradiol stock solution. Then you take out 100 uL from tube # 1 and add it to 300 uL assay buffer in tube #2, this is repeated for tubes #3-6 (for tube #3 remove 100 uL from tube #2, etc.). In tube #1 you end up with a final...
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water...
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water blanks. Your original samples is undiluted milk.
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
1 You want to make 75 mL of a dilution of disinfectant in water. How much water will you add?n
1 You want to make 75 mL of a dilution of disinfectant in water. How much water will you add?n
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Reflux procedure and report 1) You start at the same time and in same devices reflux of 40 ml of ...
Reflux procedure and report 1) You start at the same time and in same devices reflux of 40 ml of hexane and in another one 40 ml of benzene. Which device will start reflux first? Explain 2) Why reflux is a referable method of running chemical reactions that require heating? Why not just heat your reaction content in the Erlenmeyer flask? 3) Why do we turn heat off first and water only after your device is at room temperature? 4)...
Can you make up 20 ml of a 1:200 dilution from 2 ml of a 1:40...
Can you make up 20 ml of a 1:200 dilution from 2 ml of a 1:40 stock solution? (a) If so, how? If not why not? (b) If not what is the largest volume of a 1:200 you could make?
If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water...
If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water as diluent, how much 10mg/ml of BSA would you add? What would be your final concentration of BSA? If you assayed both these solutions using the Bradford assay which one would have a higher O.D.?
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
4. While filtering your product on the Buchner funnel, you washed the crystals with 15 mL of cold water. What is the purpose of washing the crystals? a. I alss hale b. Why do you think it was necessary for the water to bè cold Ttis neressam For the w becavse itiatw melt thestals Instructor Initials_ CHL 161 Experiment #8 (Rev. 1/7/2019)
4. While filtering your product on the Buchner funnel, you washed the crystals with 15 mL of cold...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
1 You want to make 75 mL of a dilution of disinfectant in water. How much water will you add?n
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Reflux procedure and report 1) You start at the same time and in same devices reflux of 40 ml of hexane and in another one 40 ml of benzene. Which device will start reflux first? Explain 2) Why reflux is a referable method of running chemical reactions that require heating? Why not just heat your reaction content in the Erlenmeyer flask? 3) Why do we turn heat off first and water only after your device is at room temperature? 4)...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
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