Question

please list 3 ways you could genetically manipulate cells to increase the amount of the transcription factor RAP?

Answer 1

1. Insert a gene of transcription factor RAP in high copy Plasmid and transfer this plasmid to host cell and select the host for positive transformation selection.

Can be done through, Transfection or Transduction

2. Increasing the levels of activation factors that increase levels of  RAP.

it can be done by Can be done through, Transfection or Transduction

3. Decreasing the levels of inhibitory factors that decrease the levels of  RAP.

it can be done by Can be done through CRISPR Cas Technique or siRNA Techniques both cleaves the protein of interest. As repressor is silenced it cannot feedback inhibit RAP protein expression.