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You speculate that a cancer drug blocks a transcription factor in human cells. You do not...

You speculate that a cancer drug blocks a transcription factor in human cells. You do not know the identity of the transcription factor but you know it binds to the DNA sequence 5’-CCTGACGTGG-3’. Briefly describe how you will identify this transcription factor using biochemical approaches.

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Here the transcription factor (TF) is reported to bind to a known sequence but, we have no information about the the TF. The TFs generally binds to distinct sites on the DNA and the TFs can then up-regulate or down-regulate transcription.An invivo technique can be used for the identification process.The TF-DNA complex which is formed invivo is at first crosslinked by formaldehyde as,formaldehyde can crosslink macromolecules and can also modify the displayed groups on the macromolecule.The TF-DNA complex will then be isolated and the bound TF will be identified by MALDI/ToF MS/MS.After isolation and identification of the TF the binding of the TF with the given sequence will be confirmed by gel mobility shift assay.

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