Question

If the volume of DNA solution was 0.5 mL, calculate the yield if:

(a) the Absorbance reading at 260nm from a 1:100 dilution = 0.459

(b) the Absorbance reading at 260nm from a 1:100 dilution = 0.167

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Answer 1

Ans: The volume of DNA solution was 0.5 mL.

(a) DNA concentration (µg/ml) = OD of DNA at 260nm  × dilution factor × 50µg/ml

= 0.459 x 1/100 x 50

= 0.2295 µg/ml

DNA yield (µg) = DNA concentration × total sample volume (ml)

= 0.2295 x 0.5

= 0.1147 µg

(b)  DNA concentration (µg/ml) = OD of DNA at 260nm  × dilution factor × 50µg/ml

= 0.167 x 1/100 x 50

= 0.0835 µg/ml

DNA yield (µg) = DNA concentration × total sample volume (ml)

= 0.0835 x 0.5

= 0.0417 µg