What is the function of 16S rRNA in bacteria? Be specific and complete in your answer.
• 16S ribosomal RNA (or 16S rRNA) is the component of the 30S small
subunit of a prokaryotic ribosome.
• Role of 16s rRNA in bacterial translation.
-16s rRNA has a important role in initiation of translation in
prokaryotes.
-The 3' end of 16s rRNA complexes with an initiating signal site on
m-RNA and helps in position the m-RNA on ribosome.(The 3' end
contains the anti-Shine-Dalgarno sequence, which binds upstream to
the AUG start codon on the mRNA).
-16s rRNA binds initiation factor-3 and helps in initiation of
prokaryotic translation.
• 16s rRNA used for classification of bacteria
- 16s rRNA is a highly conserved structure in bacteria. That means
it has obtained very less mutations over the years.
-Thus, it is due to this evolutionary conserved nature of 16s rRNA,
it has found use as a marker for the classification of
bacteria.
• Structural Roles of 16s rRNA
-It has a structural role, acting as a scaffold maintaining the
positions of the ribosomal proteins.
What is the function of 16S rRNA in bacteria? Be specific and complete in your answer.
In 16s rRNA sequencing why would someone choose to look at HV region 4 to try to find the soil microbiota bacteria profile opposed to another HV region.
3. What would be the effect of truncating the 3'end of the 16S rRNA of a prokaryote? 4. List instances in which GTP plays a role in protein synthesis.
In the protocol we followed, 16S rRNA gene segments were amplified by PCR, then individually ligated into a vector, transformed and propagated in bacteria, and 9 bacterial “clones” sequenced by the Sanger method. Each clone had a unique sequence we subsequently compared. If we had used an Illumina Next Generation Sequencing method, would the “cloning” of individual fragments have been necessary?
Find the two primer sequences in the 16S rRNA genes shown below. Underline the sequences and show any mismatches. Determine the size of the PCR fragment by finding the number of bases between the two primers. AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA Number of bases in PCR fragment:____________________________ >NR_112116.2 Bacillus subtilis strain IAM 12118 16S ribosomal RNA, complete sequence TTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTGGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT Number of bases in PCR fragment:______________________________
What would the correct answer for this question be and why? And
why would the other choices be wrong?
We were unable to transcribe this imageQuestion 85 Which of the following would most strongly reduce the strength of a real-time colony PCR? O A. 16S rRNA has minor SNPs between different species of bacteria. O B. Consistently running the PCR amplification for one O C. Different species of bacteria have different numbers O D. Different species of bacteria express 16S...
In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other genes might help us to identify specific bacteria? In other words, what traits could be sequenced that are unique to bacteria?
Bacteria in the genus Rhizobium and in the genus Bartonella are believed to have come from the same common ancestor based on 16S rRNA and they are grouped in the same order (Rhizobiales). However, bacteria in the rhizobium group but not Bartonella can convert nitrogen from the air to ammonia even though both of them came from the same ancestor. How is this possible and what could have happened to bacteria in the group rhizobium to enable it to convert...
numbers in bacteria. In contrast, the gene for ribosomal 1. (1 pts) rRNA genes are usually present on large numbers in bacteria. In contrast, the ge proteins are few. Explain why. 2. (2 pts) Name 4 different molecules that physically interact with tRNA 3. (1 pts) Would a substitution within a codon for Trp always change the resulting protein sequence? Explain your answer. 4. (1 pts) How does the ribosome know to bind to the first AUG and not to...
Once a bacterial 16s rRNA gene has been amplified, it is possible to have the nucleotide sequence of the DNA determined. There are many different techniques for this. In lab, you isolated and amplified DNA from a known organism (Escherichia coli), but what if you had to identify an unknown organism? You would send the sample away for sequencing and then once you got the results you would “BLAST” the sequence. Using the Standard Nucleotide BLAST on NCBI and the...
A: The gene that codes for the rho protein in a specific species of bacteria is deleted. What function is likely lost because of this deletion? Be specific. B: What role does the TBP (TATA-Binding Protein) play in transcriptional initiation? C: What is the purpose of having a 5’ CAP on eukaryotic mRNA? D: Where do processed mRNA’s go after leaving the nucleus and how? Please answer and explain E: Explain the role of snRNPs in RNA processing. Be specific.