What effect does glucose have on β-galactosidase expression?
What effect does glucose have on β-galactosidase expression?
Homework Answers
Lac operon is a catabolic operon. It
contains three structural genes, LacZ, LacY, and LacA.
Lac operon is expressed only when glucose is absent and lactose is
absent.
In the absence of lactose, lac
repressor binds to the operator and excludes the RNA polymerase
binding to the promoter.
When lactose is present, it binds to the lac repressor and makes it
unable to binds to the operator. Now, the promoter can be occupied
by RNA polymerase to mediate transcription.
When glucose levels are low, cAMP
concentration increases which lead to the activation of CAP. The
activated CAP promotes the recruitment of RNA polymerase to the
promoter.
When glucose levels are high, cAMP levels go down. So, CAP cannot
be activated to recruit RNA polymerase to the promoter.
One of the Escherichia coli mutants is always producing β-galactosidase. What is the term for this...
One of the Escherichia coli mutants is always producing β-galactosidase. What is the term for this type of expression? In this experiment we have a wild type lac gene, mutated lacI gene, and a mutated lacZ gene. Thank you!
β-Galactosidase (β-gal) is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. A 0.360...
β-Galactosidase (β-gal) is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. A 0.360 gram sample of β-galactosidase is dissolved in water to make 0.108 L of solution, and the osmotic pressure of the solution at 25 °C is found to be 0.709 mbar. Calculate the molecular mass of β-galactosidase.
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into...
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into monomers of galactose. A population of bacteria were found with an amino acid mutation within the active site of β-galactosidase protein, and these bacteria were unable to breakdown β-galactoside polymers. A) How would you identify a population of bacteria with mutant β.galac tosidase in terms of the relative concentration of β-galactoside and galactose in their cells? B.) Explain how the amino acid mutation could...
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
The enzyme b-galactosidase converts the disaccharide lactose into? A. allolactose B. Glucose C. allolactose and glucose...
The enzyme b-galactosidase converts the disaccharide lactose into? A. allolactose B. Glucose C. allolactose and glucose D. allolactose, glucose, galactose E. sucrose and glucose
At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2...
At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2 marks)
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase....
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase. Identify the incorrect statement. a. The E. coli could be a lacZ mutant b. The E. coli may have a mutated LacI protein that cannot bind to the operator c. The E. coli may have a mutated LacI protein that cannot bind to the inducer d. The growth medium may have a high amount of glucose e. The E. coli may have a mutated...
Your Name: 1. (continued) are provided with a tube containing an junknown unit concentration of β-galactosidase....
Your Name: 1. (continued) are provided with a tube containing an junknown unit concentration of β-galactosidase. You dilute the enzyme in the appropriate buffer and perforin enzyme assay in a total volume of 1.0 ml. Following 3 minutes incubation at 37°C, the following data was obtained after blanking the instrument with a tube not containing enzyme and assaying absorbance in a 1.0 ml cuvette. tube # β-galactosidase volume added 3 3 | 5 μ1 , s μ1-1-1 Abs@410 m 0.73...
what is the effect of adding glucose in a mideum? does it affect its sensitivity or...
what is the effect of adding glucose in a mideum? does it affect its sensitivity or specificity? explain.
21. What effect does high levels of ascorbic acid have on the following urinalysis tests? Test...
21. What effect does high levels of ascorbic acid have on the following urinalysis tests? Test Effect Clinitest Glucose Blood Bilirubin Nitrite Leukocyte esterase
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into monomers of galactose. A population of bacteria were found with an amino acid mutation within the active site of β-galactosidase protein, and these bacteria were unable to breakdown β-galactoside polymers. A) How would you identify a population of bacteria with mutant β.galac tosidase in terms of the relative concentration of β-galactoside and galactose in their cells? B.) Explain how the amino acid mutation could...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Your Name: 1. (continued) are provided with a tube containing an junknown unit concentration of β-galactosidase. You dilute the enzyme in the appropriate buffer and perforin enzyme assay in a total volume of 1.0 ml. Following 3 minutes incubation at 37°C, the following data was obtained after blanking the instrument with a tube not containing enzyme and assaying absorbance in a 1.0 ml cuvette. tube # β-galactosidase volume added 3 3 | 5 μ1 , s μ1-1-1 Abs@410 m 0.73...
21. What effect does high levels of ascorbic acid have on the following urinalysis tests? Test Effect Clinitest Glucose Blood Bilirubin Nitrite Leukocyte esterase
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